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Gene/Protein Characteristic Table for FLJ00002

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Product ID	ORK01029
Accession No	AK000002
Clone name	as00002
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5023 bp) Predicted protein sequence (1513 aa)
HaloTag ORF Clone	FHC01029
Flexi ORF Clone	FXC01029
Source	Human spleen
Rouge ID	mFLJ00002 by Kazusa Mouse cDNA Project

Length: 5023 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1513 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001140	306	572	PF00664	ABC transporter
	IPR003439	647	821	PF00005	ABC transporter
	IPR001140	906	1219	PF00664	ABC transporter
	IPR003439	1294	1476	PF00005	ABC transporter
HMMSmart	IPR003593	646	830	SM00382	AAA ATPase
	IPR003593	1293	1477	SM00382	AAA ATPase
ProfileScan	NULL	53	454	PS50319	NULL
	IPR001687	649	671	PS50101	ATP/GTP-binding site motif A (P-loop)
	IPR003439	747	818	PS50100	ABC transporter
	IPR001687	1296	1321	PS50101	ATP/GTP-binding site motif A (P-loop)
	IPR003439	1403	1473	PS50100	ABC transporter
ScanRegExp	IPR003439	747	761	PS00211	ABC transporter
	IPR003439	1403	1417	PS00211	ABC transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	49	CFTQLVLSALPHALLAVLSACYL	71	SECONDARY	23
	2	90	RLAASFLLSVFPLLDLLPVALPP	112	PRIMARY	23
	3	121	LEVLAGCVAAVAWISHSLALWVL	143	PRIMARY	23
	4	155	LALALVALLPAPALVLTVLWHCQ	177	PRIMARY	23
	5	191	ARLCLLILQLAALLAYALGWAAP	213	PRIMARY	23
	6	309	LGLLKLVGTMLGFSGPLLLSLLV	331	PRIMARY	23
	7	341	LSHGLLYALGLAGGAVLGAVLQN	363	SECONDARY	23
	8	437	YQQVGVAFVGGLILALLLVPVNK	459	PRIMARY	23
	9	525	AACVYLWAALPVVISIVIFITYV	547	PRIMARY	23
	10	562	LALVRMLILPLNNFPWVINGLLE	584	SECONDARY	23
	11	900	AVGQGLALAILFSLLLMQATRNA	922	PRIMARY	23
	12	949	PASMGLFSPQLLLFSPGNLYIPV	971	SECONDARY	23
	13	987	FYLTVYATIAGVNSLCTLLRAVL	1009	PRIMARY	23
	14	1070	NAAGLLGLLAVLGSGLPWLLLLL	1092	PRIMARY	23
	15	1175	RLQLMGAAVVSAIAGIALVQHQ	1196	SECONDARY	22

RT-PCR-ELISA

Experimental conditions

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