FLJ00046

Product ID	ORK04892
Accession No	AK024454
Clone name	as00046
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4352 bp) Predicted protein sequence (169 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 169 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB15744	3.1e-72	100.0	FLJ00046 protei...	Homo sapiens
BAB15716	2.9e-71	99.4	FLJ00015 protei...	Homo sapiens
AAC05172	3.2e-71	99.4	R29368_2 [Homo ...	Homo sapiens
NP_690882	5.1e-71	99.4	egf-like module...	Homo sapiens
NP_690885	5.3e-71	99.4	egf-like module...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK024426	6.3e-77	99.4	FLJ00015
AB018329	4.7e-24	41.4	KIAA0786
AB020628	2e-23	44.5	KIAA0821
AB018311	3.3e-23	40.9	KIAA0768
D87469	4.1e-13	34.3	KIAA0279

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000832	1	131	PF00002	G-protein coupled receptor family 2 (secretin-like)
ProfileScan	IPR000832	1	128	PS50261	G-protein coupled receptor family 2 (secretin-like)

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	36	GPVCAIFSVNLVLFLVTLWILK	57	PRIMARY	22
	2	80	ATAQLFILGCTWCLGILQVGPAA	102	PRIMARY	23
	3	108	LFTIINSLQGVFIFLVYCLLSQQ	130	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test