Gene/Protein Characteristic Table for KIAA0279
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Product ID ORK04527
Accession No D87469
Description cadherin, EGF LAG seven-pass G-type receptor 2
Clone name ff05612
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (10267 bp)
Predicted protein sequence (2854 aa)
Source Human fetal brain
Rouge ID mKIAA0279 by Kazusa Mouse cDNA Project
Note We replaced ha06133, former representative clones for KIAA0279 with ff05612. (2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 10267 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1702 bp
Genome contig ID gi89161185f_109494432
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
CCCCATACTACTGAATAAACTAGTTCTGTGCGGGT
Flanking genome sequence
(125465 - 125514)
----+----*----+----*----+----*----+----*----+----*
ACAGCACTGTCACTGTCCGCTTCTGTGTGGTACCCTGTCCTCCCACAGGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 109594432 109619895 34 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 2854 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9HCU4 0 100.0 Cadherin EGF LA...
Homo sapiens
EAW56372 0 100.0 cadherin, EGF L...
Homo sapiens
XP_537042 0 97.1 similar to cadh...
Canis lupus fam...
XP_001494282 0 97.3 cadherin, EGF L...
Equus caballus
XP_618390 0 96.5 similar to cadh...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB011536 9.4e-51 43.5 KIAA0812
AB058715 1.3e-25 35.2 KIAA1812
AB018311 1.3e-23 32.1 KIAA0768
AB011160 2.4e-22 31.3 KIAA0588
AB160984 3.6e-22 26.1 KIAA0811
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002126 161 180 PR00205 Cadherin
IPR002126 220 249 PR00205 Cadherin
IPR002126 293 305 PR00205 Cadherin
IPR002126 520 539 PR00205 Cadherin
IPR002126 539 552 PR00205 Cadherin
IPR002126 694 720 PR00205 Cadherin
IPR002126 728 745 PR00205 Cadherin
IPR000832 2309 2333 PR00249 GPCR
IPR000832 2372 2395 PR00249 GPCR
IPR000832 2410 2435 PR00249 GPCR
IPR000832 2452 2477 PR00249 GPCR
HMMPfam IPR002126 117 211 PF00028 Cadherin
IPR002126 225 321 PF00028 Cadherin
IPR002126 335 427 PF00028 Cadherin
IPR002126 441 532 PF00028 Cadherin
IPR002126 546 634 PF00028 Cadherin
IPR002126 648 737 PF00028 Cadherin
IPR002126 751 843 PF00028 Cadherin
IPR002126 857 945 PF00028 Cadherin
IPR006209 1163 1216 PF00008 EGF-like
IPR006209 1223 1254 PF00008 EGF-like
IPR006209 1263 1296 PF00008 EGF-like
IPR012680 1326 1485 PF02210 Laminin G
IPR006209 1509 1540 PF00008 EGF-like
IPR012680 1575 1701 PF02210 Laminin G
IPR006209 1728 1759 PF00008 EGF-like
IPR006209 1763 1797 PF00008 EGF-like
IPR002049 1855 1888 PF00053 EGF-like
IPR001879 1904 1961 PF02793 Hormone receptor
IPR000203 2246 2294 PF01825 GPS
IPR000832 2304 2547 PF00002 GPCR
HMMSmart IPR002126 134 218 SM00112 Cadherin
IPR002126 242 328 SM00112 Cadherin
IPR002126 352 434 SM00112 Cadherin
IPR002126 458 539 SM00112 Cadherin
IPR002126 563 641 SM00112 Cadherin
IPR002126 665 744 SM00112 Cadherin
IPR002126 768 850 SM00112 Cadherin
IPR002126 874 952 SM00112 Cadherin
IPR002126 980 1056 SM00112 Cadherin
IPR006210 1162 1217 SM00181 EGF
IPR001881 1219 1255 SM00179 EGF-like calcium-binding
IPR006210 1222 1255 SM00181 EGF
IPR006210 1262 1297 SM00181 EGF
IPR001881 1263 1297 SM00179 EGF-like calcium-binding
IPR001791 1318 1485 SM00282 Laminin G
IPR001881 1505 1541 SM00179 EGF-like calcium-binding
IPR006210 1508 1541 SM00181 EGF
IPR001791 1567 1701 SM00282 Laminin G
IPR001881 1726 1760 SM00179 EGF-like calcium-binding
IPR006210 1727 1760 SM00181 EGF
IPR006210 1762 1798 SM00181 EGF
IPR001881 1764 1798 SM00179 EGF-like calcium-binding
IPR002049 1855 1900 SM00180 EGF-like
IPR001879 1903 1965 SM00008 Hormone receptor
IPR000203 2246 2299 SM00303 GPS
ProfileScan IPR002126 113 220 PS50268 Cadherin
IPR002126 221 330 PS50268 Cadherin
IPR002126 331 436 PS50268 Cadherin
IPR002126 437 541 PS50268 Cadherin
IPR002126 542 643 PS50268 Cadherin
IPR002126 644 746 PS50268 Cadherin
IPR002126 747 852 PS50268 Cadherin
IPR002126 853 954 PS50268 Cadherin
IPR002126 973 1077 PS50268 Cadherin
IPR000742 1159 1217 PS50026 EGF-like
IPR002049 1205 1265 PS50027 EGF-like
IPR000742 1219 1255 PS50026 EGF-like
IPR000742 1259 1297 PS50026 EGF-like
IPR001791 1298 1502 PS50025 Laminin G
IPR000742 1505 1541 PS50026 EGF-like
IPR001791 1545 1722 PS50025 Laminin G
IPR000742 1724 1760 PS50026 EGF-like
IPR000742 1761 1798 PS50026 EGF-like
IPR002049 1855 1902 PS50027 EGF-like
IPR001879 1887 1960 PS50227 Hormone receptor
IPR000203 2247 2299 PS50221 GPS
IPR000832 2307 2544 PS50261 GPCR
ScanRegExp IPR002126 208 218 PS00232 Cadherin
IPR002126 318 328 PS00232 Cadherin
IPR002126 424 434 PS00232 Cadherin
IPR002126 529 539 PS00232 Cadherin
IPR002126 734 744 PS00232 Cadherin
IPR002126 840 850 PS00232 Cadherin
IPR002126 942 952 PS00232 Cadherin
IPR013032 1205 1216 PS00022 EGF-like region
IPR013032 1205 1216 PS01186 EGF-like region
IPR013032 1243 1254 PS00022 EGF-like region
IPR013032 1243 1254 PS01186 EGF-like region
IPR000152 1520 1531 PS00010 Aspartic acid and asparagine hydroxylation site
IPR013032 1529 1540 PS00022 EGF-like region
IPR013032 1529 1540 PS01186 EGF-like region
IPR000152 1739 1750 PS00010 Aspartic acid and asparagine hydroxylation site
IPR013032 1748 1759 PS00022 EGF-like region
IPR013032 1748 1763 PS01186 EGF-like region
IPR013032 1786 1797 PS00022 EGF-like region
IPR013032 1874 1885 PS00022 EGF-like region
IPR002049 1874 1907 PS01248 EGF-like

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 2309 TLTYVALGVTLAALLLTFFFLTL 2331 PRIMARY 23
2 2345 NLTAALGLAQLVFLLGINQADLP 2367 SECONDARY 23
3 2369 ACTVIAILLHFLYLCTFSWALLE 2391 PRIMARY 23
4 2410 MRFYYMLGWGVPAFITGLAVGL 2431 SECONDARY 22
5 2451 IWSFAGPVAFAVSMSVFLYILAA 2473 PRIMARY 23
6 2493 LQPSFAVLLLLSATWLLALLSVN 2515 PRIMARY 23
7 2526 ATCNCIQGPFIFLSYVVLSKEV 2547 SECONDARY 22
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name Genebridge 4
Primer_f TGCTGGATGCTAACTTGATAC
Primer_r GTTTATTCAGTAGTATGGGGC
PCR product length 112 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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