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Order Kazusa clone(s) from : |
| Product ID | ORK00767 |
|---|---|
| Accession No | AB033032 |
| Description | plexin B3, transcript variant 1 |
| Clone name | fg04331 |
| Vector information | |
| cDNA sequence | DNA sequence (6145 bp) Predicted protein sequence (1912 aa) |
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HaloTag ORF Clone |
FHC00767
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| Flexi ORF Clone | FXC00767 |
| Source | Human fetal brain |
| Rouge ID |
mKIAA1206
by Kazusa Mouse cDNA Project
|
Length: 6145 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 301 bp |
|---|---|
| Genome contig ID | gi89161218f_152582905 |
| PolyA signal sequence (AATAAA,-29) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (115086 - 115135) |
----+----*----+----*----+----*----+----*----+----* |
Length: 1912 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001627 | 57 | 150 | PF01403 | Semaphorin/CD100 antigen |
| IPR002165 | 476 | 529 | PF01437 | Plexin | |
| IPR002165 | 790 | 836 | PF01437 | Plexin | |
| IPR002909 | 838 | 927 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 930 | 1014 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1018 | 1147 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1162 | 1246 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR013548 | 1329 | 1879 | PF08337 | Plexin cytoplasmic region | |
| HMMSmart | IPR001627 | 57 | 458 | SM00630 | Semaphorin/CD100 antigen |
| IPR003659 | 476 | 529 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 623 | 685 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 790 | 836 | SM00423 | Plexin/semaphorin/integrin | |
| IPR002909 | 837 | 928 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 929 | 1015 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1017 | 1148 | SM00429 | Cell surface receptor IPT/TIG | |
| ProfileScan | IPR001627 | 34 | 474 | PS51004 | Semaphorin/CD100 antigen |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 24 | RWPPFGLCLLLLLLSPPPLPLTG | 46 | SECONDARY | 23 | 2 | 1261 | VGMGAAVLIAAVLLLTLMYR | 1280 | PRIMARY | 20 |
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RT-PCR-ELISA
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Experimental conditions| Primer_f | TTCATTGACTCCTGTACCACC |
|---|---|
| Primer_r | AGTTCATCTCCTGGTAGCTCG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 6
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TTCATTGACTCCTGTACCACC |
| Primer_r | AGTTCATCTCCTGGTAGCTCG |
| PCR product length | 160(400) bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |