KIAA0812

Product ID	ORK06860
Accession No	AB011536
Description	cadherin, EGF LAG seven-pass G-type receptor 3
Clone name	hg01044
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5832 bp) Predicted protein sequence (1364 aa)
Source	Human adult brain
Rouge ID	mKIAA0812 by Kazusa Mouse cDNA Project

Length: 5832 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1736 bp
Genome contig ID	gi89161205r_48548906
PolyA signal sequence (AATAAA,-15)	+--------+--------+----*----+---- AAGCCGCGCTCTGTTTTGGGAATAAACTTCTATAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAACAGTTGTTGCCTTTTCCTGTTGGGGGCTGGGGAGGTAGGATGGAAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	r	48648906	48664378	24	99.9	Perfect prediction

Length: 1364 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAA32464	100.0	MEGF2 [Homo sap...	Homo sapiens
EAW64914	99.6	cadherin, EGF L...	Homo sapiens
Q9NYQ7	99.6	Cadherin EGF LA...	Homo sapiens
NP_001398	99.6	cadherin EGF LA...	Homo sapiens
O88278	93.4	Cadherin EGF LA...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D87469	6.5e-42	43.5	KIAA0279
AB020628	2.4e-16	24.2	KIAA0821
AB018329	3.1e-15	26.2	KIAA0786
AB018311	4.5e-15	29.8	KIAA0768
AB011122	1.4e-09	22.0	KIAA0550

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000832	593	617	PR00249	GPCR
	IPR000832	656	679	PR00249	GPCR
	IPR000832	694	719	PR00249	GPCR
	IPR000832	736	761	PR00249	GPCR
	IPR000832	805	826	PR00249	GPCR
HMMPfam	IPR006209	2	28	PF00008	EGF-like
	IPR006209	32	66	PF00008	EGF-like
	IPR002049	124	157	PF00053	EGF-like
	IPR001879	173	235	PF02793	Hormone receptor
	IPR000203	528	576	PF01825	GPS
	IPR000832	588	830	PF00002	GPCR
HMMSmart	IPR006210	1	29	SM00181	EGF
	IPR006210	31	67	SM00181	EGF
	IPR002049	124	169	SM00180	EGF-like
	IPR001879	172	239	SM00008	Hormone receptor
	IPR000203	528	581	SM00303	GPS
ProfileScan	IPR000742	1	26	PS50026	EGF-like
	IPR000742	29	67	PS50026	EGF-like
	IPR002049	124	171	PS50027	EGF-like
	IPR001879	156	234	PS50227	Hormone receptor
	IPR000203	529	581	PS50221	GPS
	IPR000832	591	827	PS50261	GPCR
ScanRegExp	IPR000152	8	19	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR013032	17	28	PS00022	EGF-like region
	IPR013032	17	32	PS01186	EGF-like region
	IPR013032	55	66	PS00022	EGF-like region
	IPR013032	55	66	PS01186	EGF-like region
	IPR013032	143	154	PS00022	EGF-like region
	IPR002049	143	176	PS01248	EGF-like
	IPR000832	815	830	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	592	AVFTHVVVAVSVAALVLTAAILL	614	PRIMARY	23
	2	625	GIHANVAAALGVAELLFLLGIHR	647	SECONDARY	23
	3	656	AVAILLHYFFLSTFAWLFVQGLH	678	PRIMARY	23
	4	694	MRFYHALGWGVPAVLLGLAVGL	715	PRIMARY	22
	5	738	FAGPVVLVIVMNGTMFLLAART	759	PRIMARY	22
	6	776	LRSSFLLLLLVSASWLFGLLAVN	798	PRIMARY	23
	7	808	HAGLCGLQGLAVLLLFCVLNAD	829	PRIMARY	22

Primer_f
Primer_r
PCR conditions	°C sec °C sec cycles

Panel name	GeneBridge 4
Primer_f	TCGGTAAGGAGAGTTTGGGTC
Primer_r	CAGACACTCCTCACCCACCAC
PCR product length	124 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles