KIAA0713

Product ID	ORK06928
Accession No	AB018256
Description	sorting nexin 13
Clone name	hg04739
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6817 bp) Predicted protein sequence (945 aa)
Source	Human adult brain
Rouge ID	mKIAA0713 by Kazusa Mouse cDNA Project

Length: 6817 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3977 bp
Genome contig ID	gi89161213r_17698991
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TTAATTTTATATCAATAAAATAAAATACTTTCTCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTTTGGAGCTTCTGTATTTCCTATAGTTTTCTGACTGTCCATTTTATC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	r	17798991	17946616	25	99.4	Perfect prediction

Length: 945 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001103565	98.8	sorting nexin 1...	Macaca mulatta
XP_532486	96.4	similar to Sort...	Canis lupus fam...
EAW93697	99.9	sorting nexin 1...	Homo sapiens
Q9Y5W8	99.8	Sorting nexin-1...	Homo sapiens
BAE21613	96.9	unnamed protein...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023192	8.4e-06	37.4	KIAA0975

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000342	486	570	PD001580	Regulator of G protein signalling
HMMPfam	IPR003114	154	341	PF02194	PX-associated
	IPR000342	497	553	PF00615	Regulator of G protein signalling
	IPR001683	631	744	PF00787	Phox-like
	IPR013937	860	943	PF08628	Sorting nexin
HMMSmart	IPR013996	154	341	SM00313	PX-associated
	IPR000342	430	570	SM00315	Regulator of G protein signalling
	IPR001683	630	744	SM00312	Phox-like
ProfileScan	IPR003114	154	341	PS51207	PX-associated
	IPR000342	430	553	PS50132	Regulator of G protein signalling
	IPR001683	627	748	PS50195	Phox-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	EGAVAAVGGGPSSFRCCYGCCHE	44	SECONDARY	23
	2	56	VIMLTEASLSIWGWGSLGIVLFL	78	PRIMARY	23
	3	88	YLTFYILCFVGGGLVVTLLFGKT	110	PRIMARY	23

Primer_f	AATCCACCACTACCAAGAGAG
Primer_r	CACTCTGGCACTTTGTCATTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AACATCTCTTTCAGGTCTGGA
Primer_r	CCCTAAGAGCAAAACACAGCC
PCR product length	208 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles