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Order Kazusa clone(s) from : |
| Product ID | ORK04232 |
|---|---|
| Accession No | AB011120 |
| Description | attractin |
| Clone name | hh00769 |
| Vector information | |
| cDNA sequence | DNA sequence (5632 bp) Predicted protein sequence (452 aa) |
| Source | Human adult brain |
| Rouge ID |
mKIAA0548
by Kazusa Mouse cDNA Project
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Length: 5632 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 452 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR002165 | 9 | 84 | PF01437 | Plexin |
| IPR002049 | 86 | 129 | PF00053 | EGF-like | |
| IPR002049 | 132 | 178 | PF00053 | EGF-like | |
| HMMSmart | IPR003659 | 9 | 84 | SM00423 | Plexin/semaphorin/integrin |
| IPR002049 | 86 | 129 | SM00180 | EGF-like | |
| IPR002049 | 132 | 178 | SM00180 | EGF-like | |
| ProfileScan | IPR002049 | 86 | 131 | PS50027 | EGF-like |
| IPR002049 | 132 | 180 | PS50027 | EGF-like | |
| ScanRegExp | IPR002049 | 100 | 134 | PS01248 | EGF-like |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 273 | NITFFVYVSNFTWPIKIQIAFSQ | 295 | SECONDARY | 23 | 2 | 301 | DLVQFFVTFFSCFLSLLLVAAVV | 323 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | AAATGCACAGACCTCCCTTAG |
|---|---|
| Primer_r | ATTTCATTGCGGGGATTGTGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 20
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AAATGCACAGACCTCCCTTAG |
| Primer_r | ATTTCATTGCGGGGATTGTGG |
| PCR product length | 126 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |