KIAA0758

Product ID	ORK00625
Accession No	AB018301
Description	adhesion G protein-coupled receptor F5, transcript variant 1
Clone name	hh15259
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5655 bp) Predicted protein sequence (1370 aa)
HaloTag ORF Clone	FHC00625
Flexi ORF Clone	FXC00625
Source	Human adult brain
Rouge ID	mKIAA0758 by Kazusa Mouse cDNA Project
Note	We replaced hk04615, former representative clones for KIAA0758 with hh15259. (2005/08/06)

Length: 5655 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1387 bp
Genome contig ID	gi89161210r_46828301
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- TTTGGTGTGTATTTCATTAAAAAATATAAATATTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATGAAAATTGCACGCATATTAGAGTTAACCATGTACTATTGATACAGCAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	r	46928301	46997611	21	99.4	Perfect prediction

Length: 1370 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001140861	99.3	G-protein coupl...	Pan troglodytes
EAX04309	99.9	G protein-coupl...	Homo sapiens
Q8IZF2	100.0	Probable G-prot...	Homo sapiens
EAX04307	99.9	G protein-coupl...	Homo sapiens
AAS21061	99.9	G protein coupl...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	5.3e-09	25.6	KIAA0768
AB020628	3.2e-08	23.7	KIAA0821
AB018329	7.2e-08	24.6	KIAA0786
D87469	9e-06	27.1	KIAA0279
AB011536	0.00034	20.9	KIAA0812

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008078	84	102	PR01695	Ig-hepta receptor
	IPR008078	163	188	PR01695	Ig-hepta receptor
	IPR008078	400	417	PR01695	Ig-hepta receptor
	IPR008078	422	448	PR01695	Ig-hepta receptor
	IPR008078	451	475	PR01695	Ig-hepta receptor
	IPR008078	690	711	PR01695	Ig-hepta receptor
	IPR008078	724	737	PR01695	Ig-hepta receptor
	IPR000832	1038	1062	PR00249	GPCR
	IPR008078	1062	1074	PR01695	Ig-hepta receptor
	IPR000832	1112	1135	PR00249	GPCR
	IPR008078	1136	1152	PR01695	Ig-hepta receptor
	IPR008078	1178	1195	PR01695	Ig-hepta receptor
	IPR000832	1197	1222	PR00249	GPCR
	IPR008078	1223	1240	PR01695	Ig-hepta receptor
	IPR000832	1241	1261	PR00249	GPCR
	IPR000832	1272	1293	PR00249	GPCR
	IPR008078	1296	1322	PR01695	Ig-hepta receptor
	IPR008078	1324	1348	PR01695	Ig-hepta receptor
HMMPfam	IPR000082	191	290	PF01390	SEA
	IPR013151	310	376	PF00047	Immunoglobulin
	IPR013151	509	571	PF00047	Immunoglobulin
	IPR000203	974	1021	PF01825	GPS
	IPR000832	1080	1297	PF00002	GPCR
HMMSmart	IPR003599	302	392	SM00409	Immunoglobulin subtype
	IPR003598	308	381	SM00408	Immunoglobulin subtype 2
	IPR003599	501	587	SM00409	Immunoglobulin subtype
	IPR003598	507	576	SM00408	Immunoglobulin subtype 2
	IPR000203	974	1026	SM00303	GPS
ProfileScan	IPR007110	291	376	PS50835	Immunoglobulin-like
	IPR007110	393	490	PS50835	Immunoglobulin-like
	IPR007110	495	585	PS50835	Immunoglobulin-like
	IPR000203	975	1026	PS50221	GPS
	IPR000832	1036	1294	PS50261	GPCR
ScanRegExp	IPR013032	144	157	PS01186	EGF-like region
ScanRegExp	IPR000832	1282	1297	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	31	TTLCLMFIVIYSSKAALNWNYES	53	SECONDARY	23
	2	1036	LDIISYVGVGFSILSLAACLVVE	1058	PRIMARY	23
	3	1076	TCIVNIAASLLVANTWFIVVAAI	1098	SECONDARY	23
	4	1114	TFFIHFFYLSVFFWMLTLGLMLF	1136	PRIMARY	23
	5	1152	KAIAFCLGYGCPLAISVITLGAT	1174	SECONDARY	23
	6	1198	AFAIPALIIVVVNITITIVVITK	1220	PRIMARY	23
	7	1239	FQISKSIGVLTPLLGLTWGFGLT	1261	SECONDARY	23
	8	1272	HIIFAILNVFQGLFILLFGCLWD	1294	PRIMARY	23

Primer_f	ATTGTGGATACTGGCCCTTGG
Primer_r	CATTAGGAGCACAGAACAACC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	ATTGTGGATACTGGCCCTTGG
Primer_r	CATTAGGAGCACAGAACAACC
PCR product length	178 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles