KIAA0550

Product ID	ORK00559
Accession No	AB011122
Description	adhesion G protein-coupled receptor B3
Clone name	hh15909
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5420 bp) Predicted protein sequence (1524 aa)
HaloTag ORF Clone	FHC00559
Flexi ORF Clone	FXC00559
Source	Human adult brain
Rouge ID	mKIAA0550 by Kazusa Mouse cDNA Project
Note	We replaced hh00851, former representative clones for KIAA0550 with hh15909. (2002/5/10)

Length: 5420 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	614 bp
Genome contig ID	gi89161210f_69302564
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- AAAATGTTGTATGGTGTAAATAAACTTTTGTCTAC
Flanking genome sequence (853556 - 853605)	----+--------+--------+--------+--------+----* ATATCAGTTTTTTAGTGCATTTTATTTTGGATTTGTTGACCAGAAATTTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	f	69402564	70156118	32	99.9	Perfect prediction

Length: 1524 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O60242	100.0	Brain-specific ...	Homo sapiens
CAH73864	99.9	brain-specific ...	Homo sapiens
BAF82385	99.8	unnamed protein...	Homo sapiens
XP_001503435	98.7	similar to Brai...	Equus caballus
XP_538991	98.5	similar to Brai...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	2.8e-25	22.8	KIAA0768
AB018329	5.7e-25	26.1	KIAA0786
AB020628	4.7e-24	25.5	KIAA0821
AB040878	1.6e-20	31.9	KIAA1445
D87469	3.6e-20	24.8	KIAA0279

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008077	57	74	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	539	551	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	559	575	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	616	635	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	658	667	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	719	740	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	881	905	PR00249	GPCR
	IPR008077	905	915	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	945	968	PR00249	GPCR
	IPR008077	969	982	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	982	1007	PR00249	GPCR
	IPR008077	1008	1025	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	1025	1050	PR00249	GPCR
	IPR000832	1100	1120	PR00249	GPCR
	IPR008077	1121	1131	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	1131	1152	PR00249	GPCR
	IPR008077	1185	1198	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	1413	1427	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	1430	1444	PR01694	Brain-specific angiogenesis inhibitor
HMMPfam	IPR000884	297	344	PF00090	Thrombospondin
	IPR000884	351	399	PF00090	Thrombospondin
	IPR000884	406	454	PF00090	Thrombospondin
	IPR000884	461	509	PF00090	Thrombospondin
	IPR001879	513	574	PF02793	Hormone receptor
	IPR000203	817	865	PF01825	GPS
	IPR000832	876	1156	PF00002	GPCR
HMMSmart	IPR000884	296	345	SM00209	Thrombospondin
	IPR000884	350	400	SM00209	Thrombospondin
	IPR000884	405	455	SM00209	Thrombospondin
	IPR000884	460	510	SM00209	Thrombospondin
	IPR001879	512	578	SM00008	Hormone receptor
	IPR000203	817	870	SM00303	GPS
ProfileScan	IPR000859	32	161	PS01180	CUB
	IPR000884	293	345	PS50092	Thrombospondin
	IPR000884	347	400	PS50092	Thrombospondin
	IPR000884	402	455	PS50092	Thrombospondin
	IPR000884	457	510	PS50092	Thrombospondin
	IPR001879	493	573	PS50227	Hormone receptor
	IPR000203	818	870	PS50221	GPS
	IPR000832	879	1153	PS50261	GPCR
ScanRegExp	IPR000832	1141	1156	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	AVRNLLIYIFSTYLLVMFGFNAA	27	PRIMARY	23
	2	881	VTLIVGSGLSCLALITLAVVYAA	903	PRIMARY	23
	3	912	RSIILINFCLSIISSNILILVG	933	SECONDARY	22
	4	943	CTTTTAFLHFFFLASFCWVLTEA	965	SECONDARY	23
	5	985	FLCLGWGLPALVVATSVGF	1003	SECONDARY	19
	6	1025	YAFVGPAAAVVLVNMVIGILVFN	1047	PRIMARY	23
	7	1101	LWSSCVVLPLLALTWMSAVLAMT	1123	PRIMARY	23
	8	1130	FQILFAVFDSLQGFVIVMVHCIL	1152	PRIMARY	23

Primer_f	ATCATTTCACATACCTCCTGC
Primer_r	GGATAAGGATGAGGAGGGGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	ATCATTTCACATACCTCCTGC
Primer_r	GGATAAGGATGAGGAGGGGAC
PCR product length	127 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles