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Order Kazusa clone(s) from : |
| Product ID | ORK05627 |
|---|---|
| Accession No | AB023168 |
| Description | neuroligin 4, Y-linked |
| Clone name | hj05306 |
| Vector information | |
| cDNA sequence | DNA sequence (4935 bp) Predicted protein sequence (679 aa) |
| Source | Human adult brain |
Length: 4935 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 2706 bp |
|---|---|
| Genome contig ID | gi89161220f_15044026 |
| PolyA signal sequence (None) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (421218 - 421267) |
----+----*----+----*----+----*----+----*----+----* |
Length: 679 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR000460 | 299 | 313 | PR01090 | Neuroligin |
| IPR000460 | 402 | 423 | PR01090 | Neuroligin | |
| IPR000460 | 432 | 450 | PR01090 | Neuroligin | |
| IPR000460 | 532 | 561 | PR01090 | Neuroligin | |
| HMMPfam | IPR002018 | 1 | 453 | PF00135 | Carboxylesterase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 538 | LSVTIAVGASLLFLNILAFAAL | 559 | PRIMARY | 22 |
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RT-PCR-ELISA
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Experimental conditions| Primer_f | TTCTGCAAGCAATGTATGACC |
|---|---|
| Primer_r | TAGACAGACAAAACCAGCATG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 6
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TTCTGCAAGCAATGTATGACC |
| Primer_r | TAGACAGACAAAACCAGCATG |
| PCR product length | 103 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |