Gene/Protein Characteristic Table for KIAA1260
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Product ID ORK00786
Accession No AB033086
Description neuroligin 4, X-linked, transcript variant 1
Clone name hh15752
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5454 bp)
Predicted protein sequence (817 aa)
Flexi ORF Clone FXC00786
Source Human adult brain
Features of the cloned cDNA sequence
Description

Length: 5454 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2539 bp
Genome contig ID gi89161218r_5718319
PolyA signal sequence
(AATATA,-18)
+----*----+----*----+----*----+----
AGAGTTCTATGAGAAAAAATATACTTGCTATCTAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAAAGAGAAAAAAGAAAAAAAATGAGAAAAAAGTAAGAAAAAAAAAAA
Features of the protein sequence
Description

Length: 817 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q8N0W4 0 100.0 Neuroligin-4, X...
Homo sapiens
XP_001138543 0 99.9 X-linked neurol...
Pan troglodytes
BAG51523 0 99.9 unnamed protein...
Homo sapiens
BAF83725 0 99.9 unnamed protein...
Homo sapiens
XP_001087740 0 99.4 similar to X-li...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023168 0 98.8 KIAA0951
AB040913 2.4e-188 77.3 KIAA1480
AB028993 1.4e-111 74.9 KIAA1070
AB037787 2.9e-109 62.7 KIAA1366
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000460 437 451 PR01090 Neuroligin
IPR000460 540 561 PR01090 Neuroligin
IPR000460 570 588 PR01090 Neuroligin
IPR000460 670 699 PR01090 Neuroligin
HMMPfam IPR002018 26 591 PF00135 Carboxylesterase
ScanRegExp IPR002018 145 155 PS00941 Carboxylesterase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 13 LLFTPVCVMLNSNVLLWLTALAI 35 PRIMARY 23
2 676 LSVTIAVGASLLFLNILAFAAL 697 PRIMARY 22
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AGTAAATACGGCTCTGTGTTC
Primer_r GATAAATAAGGGACCGAGTGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name GeneBridge 4
Primer_f AGTAAATACGGCTCTGTGTTC
Primer_r GATAAATAAGGGACCGAGTGC
PCR product length 135 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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