KIAA1070

Product ID	ORK00172
Accession No	AB028993
Description	neuroligin 1
Clone name	hj05602
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4960 bp) Predicted protein sequence (826 aa)
HaloTag ORF Clone	FHC00172
Flexi ORF Clone	FXC00172
Source	Human adult brain
Rouge ID	mKIAA1070 by Kazusa Mouse cDNA Project

Length: 4960 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1819 bp
Genome contig ID	gi89161205f_174685171
PolyA signal sequence (None)	+--------+--------+----*----+---- AAAATGTTTATTGCTGTTTTGTCTTTCTTTTTTCT
Flanking genome sequence (798438 - 798487)	----+--------+--------+--------+--------+----* AAAAAAAGAAAAAAAAGAAAAAAAGGAAAAGAAGAACAAAGAAACATGAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	f	174785171	175483607	6	99.2	Perfect prediction

Length: 826 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH32555	100.0	Neuroligin 1 [H...	Homo sapiens
XP_001166321	99.9	neuroligin 1 is...	Pan troglodytes
XP_001082770	99.3	neuroligin 1 is...	Macaca mulatta
XP_001494442	99.1	neuroligin 1 is...	Equus caballus
XP_545297	98.9	similar to neur...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037787	2.6e-111	63.0	KIAA1366
AB033086	2.1e-110	74.9	KIAA1260
AB023168	2.1e-110	75.0	KIAA0951
AB040913	1.4e-107	71.7	KIAA1480

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000460	455	469	PR01090	Neuroligin
	IPR000460	558	579	PR01090	Neuroligin
	IPR000460	588	606	PR01090	Neuroligin
	IPR000460	673	702	PR01090	Neuroligin
HMMPfam	IPR002018	36	609	PF00135	Carboxylesterase
ScanRegExp	IPR002018	154	164	PS00941	Carboxylesterase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	24	VHRGLGAPLTLCMLGCLLQAGHV	46	SECONDARY	23
SOSUI2	2	679	LSVTIAVGASLLFLNILAFAAL	700	PRIMARY	22

Primer_f	TTCATCCCCCTGTTGTTTACC
Primer_r	TCAGGATGTGCTCTTTAAGTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TGAAGATGCTGCTCCAATACA
Primer_r	TATACCTCCGATTTCTTACAG
PCR product length	106 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles