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Order Kazusa clone(s) from : |
| Product ID | ORK01605 |
|---|---|
| Accession No | AB018285 |
| Description | lysine (K)-specific demethylase 3A, transcript variant 1 |
| Clone name | hk04073s2 |
| Vector information | |
| cDNA sequence | DNA sequence (4652 bp) Predicted protein sequence (1338 aa) |
|
HaloTag ORF Clone |
FHC01605
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| Flexi ORF Clone | FXC01605 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0742
by Kazusa Mouse cDNA Project
|
| Note | We replaced hk04073s1 and hk04073, former representative clones for KIAA0742 with hk04073s2. (2013/5/10,2002/5/10) |
Length: 4652 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1338 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR013129 | 1168 | 1281 | PF02373 | Transcription factor jumonji |
| HMMSmart | IPR003347 | 1075 | 1298 | SM00558 | Transcription factor jumonji/aspartyl beta-hydroxylase |
| ProfileScan | IPR003347 | 1075 | 1298 | PS51184 | Transcription factor jumonji/aspartyl beta-hydroxylase |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | ACCTCGTATCAGCTCTGGAAC |
|---|---|
| Primer_r | CTGTCACTTCCTCCATTGCTC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 2
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | ACCTCGTATCAGCTCTGGAAC |
| Primer_r | CTGTCACTTCCTCCATTGCTC |
| PCR product length | 172 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |