KIAA0481

Product ID	ORK00088
Accession No	AB007950
Description	transmembrane and coiled-coil domain family 2, transcript variant 1
Clone name	hm00132
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3998 bp) Predicted protein sequence (732 aa)
HaloTag ORF Clone	FHC00088
Flexi ORF Clone	FXC00088
Source	Human adult brain
Rouge ID	mKIAA0481 by Kazusa Mouse cDNA Project
Note	We replaced hh01480 and hh01480s1, former representative clones for KIAA0481 with hm00132. (2002/5/10,2005/08/06)

Length: 3998 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1213 bp
Genome contig ID	gi89161185f_203363661
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- CACCTTCCTGAGAAATAAATATCCTCTAAATTTTC
Flanking genome sequence (145429 - 145478)	----+--------+--------+--------+--------+----* AAACCATCCTGTCTGGTGTTGGTTGATTAGGTTGTGTCTGTGGGAACCAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	203463661	203509088	5	99.6	Perfect prediction

Length: 732 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O75069	0	100.0	Transmembrane a...	Homo sapiens
XP_001160985	0	99.4	hypothetical pr...	Pan troglodytes
XP_001094601	0	99.0	similar to tran...	Macaca mulatta
XP_545686	5.6e-212	94.5	similar to tran...	Canis lupus fam...
Q80W04	1.1e-209	94.2	Transmembrane a...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032971	8.2e-51	55.7	KIAA1145
AB018322	4.7e-40	64.0	KIAA0779

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	667	GKFINVILALMAVLLVFVSTIAN	689	PRIMARY	23
SOSUI2	2	705	TLLVLVLFLLWKHWDSLTYLLEH	727	SECONDARY	23

Primer_f
Primer_r
PCR conditions	°C sec °C sec cycles

Panel name	GeneBridge 4
Primer_f	CTCAGGAAGGTGCACAACAGC
Primer_r	GCTCTGGAGTGTTGTGTATGG
PCR product length	146 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles