KIAA1250

Product ID	ORK05646
Accession No	AB033076
Description	kinase D-interacting substrate, 220kDa
Clone name	pf01137
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7264 bp) Predicted protein sequence (1777 aa)
HaloTag ORF Clone	FHC05646
Flexi ORF Clone	FXC05646
Source	Human brain (hippocampus)
Rouge ID	mKIAA1250 by Kazusa Mouse cDNA Project
Note	We replaced hh06271, former representative clones for KIAA1250 with pf01137. (2001/5/29)

Length: 7264 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1791 bp
Genome contig ID	gi89161199r_8686511
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- AAATAGATTTAGAAAACAATAAAAAATTGCATGCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTCTGACTCATGAATTTTTATTCACTATGATGATTCACATTTTGATTAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	r	8786511	8895181	30	99.6	Perfect prediction

Length: 1777 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9ULH0	100.0	Ankyrin repeat-...	Homo sapiens
XP_001083115	99.1	similar to kina...	Macaca mulatta
XP_532865	96.0	similar to kina...	Canis lupus fam...
XP_001083357	95.9	similar to kina...	Macaca mulatta
Q9EQG6	93.3	Ankyrin repeat-...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014597	1.3e-18	32.1	KIAA0697
AB033049	1.5e-17	34.7	KIAA1223
AB002377	1.7e-14	28.6	KIAA0379
AB023174	5.9e-12	30.2	KIAA0957
AB051515	4.7e-10	30.5	KIAA1728

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002110	44	56	PR01415	Ankyrin
FPrintScan	IPR002110	155	167	PR01415	Ankyrin
HMMPfam	IPR002110	43	75	PF00023	Ankyrin
	IPR002110	76	108	PF00023	Ankyrin
	IPR002110	109	141	PF00023	Ankyrin
	IPR002110	142	174	PF00023	Ankyrin
	IPR002110	175	207	PF00023	Ankyrin
	IPR002110	208	240	PF00023	Ankyrin
	IPR002110	241	273	PF00023	Ankyrin
	IPR002110	274	306	PF00023	Ankyrin
	IPR002110	307	339	PF00023	Ankyrin
	IPR002110	340	372	PF00023	Ankyrin
	IPR002110	373	396	PF00023	Ankyrin
	IPR011646	446	959	PF07693	KAP P-loop
HMMSmart	IPR002110	43	72	SM00248	Ankyrin
	IPR002110	76	105	SM00248	Ankyrin
	IPR002110	109	138	SM00248	Ankyrin
	IPR002110	142	171	SM00248	Ankyrin
	IPR002110	175	204	SM00248	Ankyrin
	IPR002110	208	237	SM00248	Ankyrin
	IPR002110	241	270	SM00248	Ankyrin
	IPR002110	274	303	SM00248	Ankyrin
	IPR002110	307	336	SM00248	Ankyrin
	IPR002110	340	369	SM00248	Ankyrin
	IPR002110	373	404	SM00248	Ankyrin
ProfileScan	IPR002110	23	396	PS50297	Ankyrin
	IPR002110	43	75	PS50088	Ankyrin
	IPR002110	76	108	PS50088	Ankyrin
	IPR002110	109	141	PS50088	Ankyrin
	IPR002110	142	174	PS50088	Ankyrin
	IPR002110	175	207	PS50088	Ankyrin
	IPR002110	208	240	PS50088	Ankyrin
	IPR002110	241	273	PS50088	Ankyrin
	IPR002110	274	306	PS50088	Ankyrin
	IPR002110	340	372	PS50088	Ankyrin
	IPR002110	373	396	PS50088	Ankyrin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	LQLSVKMSVLISQSVINYVEEEN	23	SECONDARY	23
	2	502	QFSWLIVFLTLLLCGGLGLLFAF	524	PRIMARY	23
	3	531	GIAVSLSFLALLYIFFIVIYFGG	553	PRIMARY	23
	4	663	LPSFVIFLFIIGCIISGITLLAI	685	PRIMARY	23
	5	693	LTVNAVLISIASVVGLAFVLNCR	715	PRIMARY	23

Primer_f	AGCCGAGTTAAGAGAATGGTC
Primer_r	CCATCTACCGTCCTAACTGTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-