FLJ00320

Product ID	ORK07526
Accession No	AK160376
Clone name	sh07586
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5731 bp) Predicted protein sequence (267 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 267 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD18719	1e-101	100.0	FLJ00320 protei...	Homo sapiens
BAG57634	2.6e-84	98.7	unnamed protein...	Homo sapiens
XP_512943	9.2e-84	93.0	hypothetical pr...	Pan troglodytes
EAW72559	2.3e-71	100.0	zinc finger pro...	Homo sapiens
AAQ62849	1e-70	99.5	truncated zinc ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB075842	8.3e-11	45.6	KIAA1962
AB007886	2.7e-10	48.5	KIAA0426
AK074116	1.3e-09	36.9	FLJ00187
AB023232	1.6e-09	46.7	KIAA1015
AB011129	2.6e-09	53.6	KIAA0557

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003309	113	208	PF02023	Transcriptional regulator SCAN
HMMSmart	IPR003309	115	227	SM00431	Transcriptional regulator SCAN
ProfileScan	IPR003309	119	197	PS50804	Transcriptional regulator SCAN

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	SGSWGGCALALCKAIALSVSSAG	31	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test