FLJ00180

Product ID	ORK05105
Accession No	AK074109
Clone name	sj01466
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4287 bp) Predicted protein sequence (499 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 499 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84935	1.1e-182	100.0	FLJ00180 protei...	Homo sapiens
Q7RTR2	2.1e-182	100.0	Protein NLRC3; ...	Homo sapiens
EAW85351	2.2e-182	100.0	NOD3 protein, i...	Homo sapiens
DAA01245	2.2e-182	100.0	TPA_inf: NOD3 [...	Homo sapiens
XP_001499317	2.8e-168	91.2	NLR family, CAR...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK090431	1.5e-55	91.3	FLJ00348
AK090476	4.5e-24	100.0	FLJ00398
AB058738	6.7e-10	25.0	KIAA1835
AB023143	1.6e-07	30.0	KIAA0926
AK074167	9e-07	26.0	FLJ00240

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	242	255	PR00019	Leucine-rich repeat
FPrintScan	IPR001611	435	448	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	129	162	PF00560	Leucine-rich repeat
	IPR001611	241	264	PF00560	Leucine-rich repeat
	IPR001611	409	436	PF00560	Leucine-rich repeat
	IPR001611	437	464	PF00560	Leucine-rich repeat
	IPR001611	465	492	PF00560	Leucine-rich repeat
HMMSmart	IPR003590	99	126	SM00368	Leucine-rich repeat
	IPR003590	127	154	SM00368	Leucine-rich repeat
	IPR003590	155	182	SM00368	Leucine-rich repeat
	IPR003590	183	210	SM00368	Leucine-rich repeat
	IPR003590	211	238	SM00368	Leucine-rich repeat
	IPR003590	239	266	SM00368	Leucine-rich repeat
	IPR003590	267	294	SM00368	Leucine-rich repeat
	IPR003590	295	322	SM00368	Leucine-rich repeat
	IPR003590	323	350	SM00368	Leucine-rich repeat
	IPR003590	351	378	SM00368	Leucine-rich repeat
	IPR003590	379	406	SM00368	Leucine-rich repeat
	IPR003590	407	434	SM00368	Leucine-rich repeat
	IPR003590	435	462	SM00368	Leucine-rich repeat
	IPR003590	463	490	SM00368	Leucine-rich repeat
ProfileScan	IPR007091	79	163	PS50503	Leucine-rich repeat
	IPR007091	164	247	PS50503	Leucine-rich repeat
	IPR007091	248	331	PS50503	Leucine-rich repeat
	IPR007091	360	443	PS50503	Leucine-rich repeat
ScanRegExp	IPR001395	120	135	PS00063	Aldo/keto reductase

Primer_f
Primer_r
PCR conditions	test