FLJ00204

Product ID	ORK05115
Accession No	AK074131
Clone name	sj04432
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4683 bp) Predicted protein sequence (573 aa)
Source	Human spleen
Rouge ID	mFLJ00204 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 573 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84957	7.5e-180	100.0	FLJ00204 protei...	Homo sapiens
EAW53865	7.8e-180	100.0	hCG27893 [Homo ...	Homo sapiens
Q8TEJ3	1e-179	100.0	SH3 domain-cont...	Homo sapiens
XP_001084626	4e-159	98.5	similar to puta...	Macaca mulatta
XP_525875	4.1e-159	98.8	similar to FLJ0...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040927	6.5e-09	42.3	KIAA1494

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001452	159	211	PD000066	SH3
BlastProDom	IPR001452	525	571	PD000066	SH3
FPrintScan	IPR001452	158	168	PR00452	SH3
	IPR001452	531	546	PR00452	SH3
	IPR001452	548	557	PR00452	SH3
	IPR001452	561	573	PR00452	SH3
HMMPfam	IPR001452	158	214	PF00018	SH3
	NULL	159	214	PF07653	NULL
	IPR001452	517	573	PF00018	SH3
	NULL	518	573	PF07653	NULL
HMMSmart	IPR001452	158	215	SM00326	SH3
HMMSmart	IPR001452	517	573	SM00326	SH3
ProfileScan	IPR001452	155	216	PS50002	SH3
ProfileScan	IPR001452	514	573	PS50002	SH3

Primer_f
Primer_r
PCR conditions	test