FLJ00231

Product ID	ORK05126
Accession No	AK074158
Clone name	sj06637
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4127 bp) Predicted protein sequence (354 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 354 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84984	9.5e-150	100.0	FLJ00231 protei...	Homo sapiens
BAC85898	8.1e-118	99.6	unnamed protein...	Homo sapiens
Q9C0H2	1.1e-117	91.2	Protein tweety ...	Homo sapiens
BAC34944	9.9e-113	85.7	unnamed protein...	Mus musculus
Q6P5F7	9.9e-113	85.7	Protein tweety ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051478	7.1e-101	91.2	KIAA1691

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	34	LQGLLETLLGYTAAIPFWRNTAV	56	SECONDARY	23
	2	65	VDLYDWYRVCLLGVLALVISWGA	87	PRIMARY	23
	3	219	LIYLALFSFVTALMFSSIVCSVP	241	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test