HUGE

Gene/Protein Characteristic Table for KIAA0192

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01589
Accession No. :	D83783
Description :	Mediator of RNA polymerase II transcription subunit 12.
HUGO Gene Name :	mediator complex subunit 12 (MED12)
Clone Name :	ha02370 [Vector Info]
Flexi ORF Clone :	pF1KSDA0192
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 6604 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 229 bp

Genome contig ID gi89161218f_70156008
PolyA signal sequence
(AATACA,-20)
+----*----+----*----+----*----+----
AGAGCTGTTGCACCCAATACACAGAGCTTCTTTGC
Flanking genome sequence
(123016 - 123065)
----+----*----+----*----+----*----+----*----+----*
AAAGGGAGTGTGCGAGTTCTGCATGTCTGGGAAGGGTGGTCTCTTGGGAG

Features of the protein sequence	Description

Length: 2124 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAD22033	100.0	thyroid hormone...	Homo sapiens
Q93074	100.0	Mediator of RNA...	Homo sapiens
EAX05315	99.9	mediator of RNA...	Homo sapiens
Q5RCU2	99.8	Mediator of RNA...	Pongo pygmaeus
XP_001088424	99.7	mediator of RNA...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB046855	2e-49	58.4	KIAA1635
AB058722	6.6e-10	32.7	KIAA1819
AB058721	2.8e-05	27.4	KIAA1818
AB028971	0.00079	29.1	KIAA1048

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 221 KLLLPLLLRYSGEFVQSAYLSR 242 SECONDARY 22

2 307 FGLSCILQTILLCCPSALVWHYS 329 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	221	KLLLPLLLRYSGEFVQSAYLSR	242	SECONDARY	22
2	307	FGLSCILQTILLCCPSALVWHYS	329	PRIMARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: X

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: CGTTGTAATACCCTTCCTGAC
Primer_r	: TTTTGGCTAGTTGCGTGAGTG
PCR product length	: 151 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	229 bp
Genome contig ID	gi89161218f_70156008
PolyA signal sequence (AATACA,-20)	+--------+--------+----*----+---- AGAGCTGTTGCACCCAATACACAGAGCTTCTTTGC
Flanking genome sequence (123016 - 123065)	----+--------+--------+--------+--------+----* AAAGGGAGTGTGCGAGTTCTGCATGTCTGGGAAGGGTGGTCTCTTGGGAG

Gene/Protein Characteristic Table for KIAA0192

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information