HUGE

Gene/Protein Characteristic Table for KIAA0294

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01064
Accession No. :	AB002292
Description :	Rho guanine nucleotide exchange factor 10.
HUGO Gene Name :	Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10)
Clone Name :	hf00223s1 [Vector Info]
Flexi ORF Clone :	pF1KA0294
Source :	Human adult brain
Note :	We replaced hf00223, former representative clones for KIAA0294 with hf00223s1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5589 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1370 bp

Genome contig ID gi51511724f_1678924
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
GCTGTCTGACTTCATCAATAAAGTATTTTTATTTT
Flanking genome sequence
(215284 - 215333)
----+----*----+----*----+----*----+----*----+----*
AAAATGCAGTAGGATGAGTTGCCTCTTTTCTGTGTCAAGTGGAAAGGGAC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 8 f 1759549 1894206 29 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 1405 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O15013	99.9	Rho guanine nuc...	Homo sapiens
EAW51484	100.0	Rho guanine nuc...	Homo sapiens
XP_528056	96.0	Rho guanine nuc...	Pan troglodytes
EAW51481	99.9	Rho guanine nuc...	Homo sapiens
EAW51483	100.0	Rho guanine nuc...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB046846	5.8e-86	46.1	KIAA1626
AB002335	1.2e-15	24.0	KIAA0337
AB028989	8.1e-09	30.0	KIAA1066
AB011088	2.2e-08	26.1	KIAA0516
AB037783	1.8e-06	25.8	KIAA1362

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR000219 461 643 PF00621 DH

HMMSmart IPR000219 461 643 SM00325 DH

ProfileScan IPR000219 457 644 PS50010 DH

ScanRegExp IPR000169 1205 1215 PS00639 Peptidase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000219	461	643	PF00621	DH
HMMSmart	IPR000219	461	643	SM00325	DH
ProfileScan	IPR000219	457	644	PS50010	DH
ScanRegExp	IPR000169	1205	1215	PS00639	Peptidase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1145 GMVISHMAVSGVGIWIAFTSGST 1167 SECONDARY 23

2 1201 TSLLVCHGLLMVGTSLGVLVALP 1223 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1145	GMVISHMAVSGVGIWIAFTSGST	1167	SECONDARY	23
2	1201	TSLLVCHGLLMVGTSLGVLVALP	1223	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: TCTTAAAATCTACCGCCAACG

Primer_r
: ACACCTCCATCTACTATTCGG

PCR conditions
: 95 °C15 sec62 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 8

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TCTTAAAATCTACCGCCAACG
Primer_r	: ACACCTCCATCTACTATTCGG
PCR product length	: 110 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1370 bp
Genome contig ID	gi51511724f_1678924
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GCTGTCTGACTTCATCAATAAAGTATTTTTATTTT
Flanking genome sequence (215284 - 215333)	----+--------+--------+--------+--------+----* AAAATGCAGTAGGATGAGTTGCCTCTTTTCTGTGTCAAGTGGAAAGGGAC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	8	f	1759549	1894206	29	99.6	Perfect prediction

Gene/Protein Characteristic Table for KIAA0294

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information