HUGE

Gene/Protein Characteristic Table for KIAA0851

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00658
Accession No. :	AB020658
Description :	suppressor of actin 1.
HUGO Gene Name :	SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L)
Clone Name :	hk06032 [Vector Info]
Flexi ORF Clone :	pF1KSDA0851
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 3572 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1739 bp

Genome contig ID gi89161205f_45605933
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
TCCATAAGTGAAAATAAAATGTCATATTCTTTTCT
Flanking genome sequence
(155973 - 156022)
----+----*----+----*----+----*----+----*----+----*
ATTATTGGTATCATTCATGTACTTTGAAAGTGATGTTTTTCTTTTTGATC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 3 f 45705893 45761904 20 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 607 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9NTJ5	100.0	Phosphatidylino...	Homo sapiens
Q5R921	99.8	Phosphatidylino...	Pongo abelii
AAH16559	99.8	SAC1 suppressor...	Homo sapiens
BAF83831	99.7	unnamed protein...	Homo sapiens
CAB66765	99.7	hypothetical pr...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023183	2.1e-53	38.0	KIAA0966
AB020717	2.7e-36	30.6	KIAA0910
AB002346	3.5e-33	30.9	KIAA0348
D87464	5e-11	28.4	KIAA0274

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR002013 77 372 PF02383 Synaptojanin

ProfileScan IPR002013 142 471 PS50275 Synaptojanin

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002013	77	372	PF02383	Synaptojanin
ProfileScan	IPR002013	142	471	PS50275	Synaptojanin

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 541 FLALPIIMVVAFSMCIICLLMAG 563 PRIMARY 23

2 574 LFWGVASIGTFFIILYNGKDFVD 596 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	541	FLALPIIMVVAFSMCIICLLMAG	563	PRIMARY	23
2	574	LFWGVASIGTFFIILYNGKDFVD	596	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TAGCTGGAAAACCCTCTGTAG

Primer_r
: CATTCCCTACAAAGCAGCCAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 3

Experimental conditions

Panel name	: UniGene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1739 bp
Genome contig ID	gi89161205f_45605933
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TCCATAAGTGAAAATAAAATGTCATATTCTTTTCT
Flanking genome sequence (155973 - 156022)	----+--------+--------+--------+--------+----* ATTATTGGTATCATTCATGTACTTTGAAAGTGATGTTTTTCTTTTTGATC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	3	f	45705893	45761904	20	100.0	Perfect prediction

Primer_f	: TAGCTGGAAAACCCTCTGTAG
Primer_r	: CATTCCCTACAAAGCAGCCAC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0851

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information