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Gene/Protein Characteristic Table for KIAA0131

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Product ID	ORK00025
Accession No	D50921
Description	Rho GTPase activating protein 4, transcript variant 2
Clone name	ha01235
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3180 bp) Predicted protein sequence (942 aa)
HaloTag ORF Clone	FHC00025
Flexi ORF Clone	FXC00025
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0131 by Kazusa Mouse cDNA Project

Length: 3180 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis). Integrity of 3' end

Length: 942 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001452	747	797	PD000066	Src homology-3
FPrintScan	IPR001452	759	774	PR00452	Src homology-3
	IPR001452	776	785	PR00452	Src homology-3
	IPR001452	787	799	PR00452	Src homology-3
HMMPfam	IPR001060	18	120	PF00611	Cdc15/Fes/CIP4
	IPR000198	517	670	PF00620	RhoGAP
	IPR001452	745	799	PF00018	Src homology-3
HMMSmart	IPR001060	18	120	SM00055	Cdc15/Fes/CIP4
	IPR000198	514	688	SM00324	RhoGAP
	IPR001452	745	800	SM00326	Src homology-3
ProfileScan	IPR001060	11	84	PS50133	Cdc15/Fes/CIP4
	IPR000198	503	691	PS50238	RhoGAP
	IPR001452	742	801	PS50002	Src homology-3

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	619	RLPAPVLVVLRYLFTFLNHLAQY	641	PRIMARY	23
	2	649	PYNLAVCFGPTLLPVPAGQD	668	SECONDARY	20

Northern blot

Experimental conditions

Chromosome No. X
Experimental conditions

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