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Order Kazusa clone(s) from : |
| Product ID | ORK00531 |
|---|---|
| Accession No | AB007876 |
| Description | leucine rich repeat transmembrane neuronal 2 |
| Clone name | hh00236 |
| Vector information | |
| cDNA sequence | DNA sequence (5572 bp) Predicted protein sequence (529 aa) |
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HaloTag ORF Clone |
FHC00531
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| Flexi ORF Clone | FXC00531 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0416
by Kazusa Mouse cDNA Project
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Length: 5572 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 529 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR001611 | 148 | 161 | PR00019 | Leucine-rich repeat |
| IPR001611 | 193 | 206 | PR00019 | Leucine-rich repeat | |
| HMMPfam | IPR001611 | 99 | 121 | PF00560 | Leucine-rich repeat |
| IPR001611 | 123 | 145 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 147 | 169 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 171 | 193 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 195 | 217 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 219 | 241 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 267 | 289 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 291 | 313 | PF00560 | Leucine-rich repeat | |
| HMMSmart | IPR003591 | 97 | 120 | SM00369 | Leucine-rich repeat |
| IPR003591 | 121 | 144 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 145 | 168 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 169 | 192 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 193 | 216 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 217 | 237 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 241 | 264 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 265 | 288 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 289 | 312 | SM00369 | Leucine-rich repeat |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 21 | PLGAPMLAAIYAMSMVLKMLPAL | 43 | SECONDARY | 23 | 2 | 436 | VITGTMALLFSFFFIIFIVFIS | 457 | PRIMARY | 22 |
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RT-PCR
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Experimental conditions| Primer_f | ATGAAAAGTATGCCCCCAAAC |
|---|---|
| Primer_r | CACTTCCTACACACAGCTATC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 5
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | ATGAAAAGTATGCCCCCAAAC |
| Primer_r | CACTTCCTACACACAGCTATC |
| PCR product length | 185 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |