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Order Kazusa clone(s) from : |
| Product ID | ORK00602 |
|---|---|
| Accession No | AB014577 |
| Description | lysine (K)-specific demethylase 4A |
| Clone name | hk02635 |
| Vector information | |
| cDNA sequence | DNA sequence (4417 bp) Predicted protein sequence (1073 aa) |
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HaloTag ORF Clone |
FHC00602
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| Flexi ORF Clone | FXC00602 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0677
by Kazusa Mouse cDNA Project
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Length: 4417 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1073 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR003349 | 24 | 69 | PF02375 | Transcription factor jumonji |
| IPR013129 | 184 | 300 | PF02373 | Transcription factor jumonji | |
| IPR001965 | 744 | 778 | PF00628 | Zinc finger | |
| HMMSmart | IPR003349 | 22 | 64 | SM00545 | Transcription factor jumonji |
| IPR003347 | 151 | 317 | SM00558 | Transcription factor jumonji/aspartyl beta-hydroxylase | |
| IPR001965 | 718 | 776 | SM00249 | Zinc finger | |
| IPR001965 | 838 | 894 | SM00249 | Zinc finger | |
| IPR002999 | 906 | 963 | SM00333 | Tudor | |
| IPR002999 | 964 | 1020 | SM00333 | Tudor | |
| ProfileScan | IPR003349 | 23 | 65 | PS51183 | Transcription factor jumonji |
| IPR003347 | 151 | 317 | PS51184 | Transcription factor jumonji/aspartyl beta-hydroxylase |
RT-PCR
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Experimental conditions| Primer_f | CCGCTGTAGTGAAAGGACGAG |
|---|---|
| Primer_r | GTTGACCCCTCTATGGACTGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 1
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CCGCTGTAGTGAAAGGACGAG |
| Primer_r | GTTGACCCCTCTATGGACTGG |
| PCR product length | 121 bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |