KIAA0735

Product ID	ORK00617
Accession No	AB018278
Description	synaptic vesicle glycoprotein 2B, transcript variant 1
Clone name	hj00943
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4948 bp) Predicted protein sequence (707 aa)
HaloTag ORF Clone	FHC00617
Flexi ORF Clone	FXC00617
Source	Human adult brain
Rouge ID	mKIAA0735 by Kazusa Mouse cDNA Project

Length: 4948 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2732 bp
Genome contig ID	gi51511731f_89470349
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- TGATCTTTTGTTTTATTAAAAATAATTAGTGAAAG
Flanking genome sequence (169171 - 169220)	----+--------+--------+--------+--------+----* AGGTGTGCCTATCTGTGAAGTTTGTAGTACATCATCCTGAGGTCATGTAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	15	f	89570334	89639518	12	99.4	Perfect prediction

Length: 707 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001099688	99.6	similar to syna...	Macaca mulatta
Q7L1I2	100.0	Synaptic vesicl...	Homo sapiens
CAG33367	99.9	SV2B [Homo sapi...	Homo sapiens
XP_545841	95.2	similar to syna...	Canis lupus fam...
XP_523160	99.3	synaptic vesicl...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018279	7.1e-164	62.4	KIAA0736
AB028977	1.6e-119	62.3	KIAA1054

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	139	674	PF07690	Major facilitator superfamily MFS_1
HMMTigr	IPR005988	1	707	TIGR01299	Synaptic vesicle protein SV2
ProfileScan	IPR007114	135	702	PS50850	Major facilitator superfamily
ScanRegExp	IPR005829	189	205	PS00216	Sugar transporter superfamily
ScanRegExp	IPR005829	231	256	PS00217	Sugar transporter superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	134	ILFFVLGLALMADGVEVFVVSFA	156	PRIMARY	23
	2	173	MLGMIVYLGMMAGAFILGGLADK	195	PRIMARY	23
	3	229	RLISGIGIGGALPIVFAYFSEFL	251	PRIMARY	23
	4	261	SWLGIFWMTGGLYASAMAWSIIP	283	SECONDARY	23
	5	300	WRVFVIVCALPCTVSMVALKFMP	322	PRIMARY	23
	6	416	ILAVVWFAMAFSYYGLTVWFPDM	438	PRIMARY	23
	7	561	LIYLVSFLGSLSVLPGNIISALL	583	SECONDARY	23
	8	591	KMIGGSMLISAVCCFFLFF	609	PRIMARY	19
	9	619	WQCLFCGTSIAAWNALDVITVEL	641	SECONDARY	23
	10	649	TAFGILNGLCKFGAILGNTIFAS	671	SECONDARY	23
	11	681	ILLAAASLVGGGLIALRLPETRE	703	PRIMARY	23

Primer_f	TCTTAAATGCCGTCACTCCTG
Primer_r	AGTGAAAAAGAAGTGGATGGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TTACCCTTCCACACAATAGCC
Primer_r	AAAACTACAACACGTCACAGC
PCR product length	110 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles