KIAA1081

Product ID	ORK00176
Accession No	AB029004
Description	ELKS/RAB6-interacting/CAST family member 1
Clone name	bg00262
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector(Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGC TGAAGCTTTC ACAAGTTTGT ACAAAAAAGC AGGCTCTTC-5' end of cDNA, GAGCTCTCAC CACTTTGTAC AAGAAAGCTG GGCGGCCGCT TTTTTTTTTT TTTTTTT-3' end of cDNA).
cDNA sequence	DNA sequence (6768 bp) Predicted protein sequence (1003 aa)
HaloTag ORF Clone	FHC00176
Flexi ORF Clone	FXC00176
Source	Human adult brain
Rouge ID	mKIAA1081 by Kazusa Mouse cDNA Project
Note	We replaced hj07146, former representative clones for KIAA1081 with bg00262. (2002/5/10)

Length: 6768 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Integrity of 3' end

Length of 3'UTR	3666 bp
Genome contig ID	gi89161190f_907208
PolyA signal sequence (None)	+--------+--------+----*----+---- ACTGCACTCCAGCCTGGGTGACAGAGACTCCATTT
Flanking genome sequence (565752 - 565801)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATGGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	f	1007208	1472958	18	99.4	Perfect prediction

Length: 1003 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAC54107	100.0	ELKS beta [Homo...	Homo sapiens
EAW88940	99.6	RAB6 interactin...	Homo sapiens
XP_868091	99.2	similar to RAB6...	Canis lupus fam...
XP_001151532	99.9	RAB6-interactin...	Pan troglodytes
BAC54109	99.6	ELKS delta [Hom...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB002376	1.7e-90	69.1	KIAA0378
AB023166	1.5e-06	23.5	KIAA0949
AB002334	1.2e-05	22.0	KIAA0336
D13629	2.3e-05	22.1	KIAA0004
AB002371	8.5e-05	20.8	KIAA0373

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Primer_f	CTTAACCAAATGTCCCACTCC
Primer_r	ACTAGACTTGCCGATGGAGGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTTAACCAAATGTCCCACTCC
Primer_r	ACTAGACTTGCCGATGGAGGG
PCR product length	170 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles