KIAA0686

Product ID	ORK05615
Accession No	AB014586
Description	adhesion G protein-coupled receptor V1
Clone name	fj02322
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3918 bp) Predicted protein sequence (1200 aa)
Source	Human fetal brain
Rouge ID	mKIAA0686 by Kazusa Mouse cDNA Project
Note	We replaced hk02975, former representative clones for KIAA0686 with fj02322. (2002/5/10)

Length: 3918 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	315 bp
Genome contig ID	gi51511721f_90042152
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TAATGAAAGTAATAATCAATAAAGCAATAGAATCT
Flanking genome sequence (453638 - 453687)	----+--------+--------+--------+--------+----* ATTTGGTATCATCCAGCTTCATTATTGATAAAGAAACATTGTGCATGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	f	90142152	90495788	17	99.7	Perfect prediction

Length: 1200 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAD55586	100.0	very large G-pr...	Homo sapiens
EAW95987	100.0	monogenic, audi...	Homo sapiens
EAW95992	100.0	monogenic, audi...	Homo sapiens
Q8WXG9	100.0	G-protein coupl...	Homo sapiens
EAW95993	100.0	monogenic, audi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	3.3e-14	22.5	KIAA0768
AB018329	1.1e-13	23.0	KIAA0786
AB020628	3.6e-13	23.9	KIAA0821
D87469	8.8e-09	22.5	KIAA0279

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003644	187	227	PF03160	Na-Ca exchanger/integrin-beta4
HMMPfam	IPR000203	746	791	PF01825	GPS
ProfileScan	IPR000203	747	796	PS50221	GPS
ProfileScan	IPR000832	799	1050	PS50261	GPCR
ScanRegExp	IPR003016	292	321	PS00189	2-oxo acid dehydrogenase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	801	EAFFTSGFICISGLCLAVLSHIF	823	SECONDARY	23
	2	833	KLLTHMMAASLGTQILFLASAYA	855	SECONDARY	23
	3	869	AVTHYLYLCQFSWMLIQSVNFWY	891	SECONDARY	23
	4	906	LFFLLSWGLPAFVVILLIVILKG	928	PRIMARY	23
	5	949	PNVYAALFTAALVPLTCLVVVFV	971	PRIMARY	23
	6	997	AEIPLILYLFALISVTWLWGGLH	1019	PRIMARY	23
	7	1028	LVLFVIFNSLQGLYVFMVYFILH	1050	PRIMARY	23

Primer_f	GTGCCTCCATTTCTTCTGCTC
Primer_r	AGGCAAAGTAAAGGCTCACAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GTGCCTCCATTTCTTCTGCTC
Primer_r	AGGCAAAGTAAAGGCTCACAG
PCR product length	164 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles