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Gene/Protein Characteristic Table for KIAA0315

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Product ID	ORK00501
Accession No	AB002313
Description	plexin B2
Clone name	hg00246
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone hg00246 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (6252 bp) Predicted protein sequence (1841 aa)
HaloTag ORF Clone	FHC00501
Flexi ORF Clone	FXC00501
Source	Human adult brain
Rouge ID	mKIAA0315 by Kazusa Mouse cDNA Project

Length: 6252 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1841 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	37	452	PF01403	Semaphorin/CD100 antigen
	IPR002165	471	522	PF01437	Plexin
	IPR002165	762	804	PF01437	Plexin
	IPR002909	806	895	PF01833	Cell surface receptor IPT/TIG
	IPR002909	898	981	PF01833	Cell surface receptor IPT/TIG
	IPR002909	986	1094	PF01833	Cell surface receptor IPT/TIG
	IPR013548	1273	1808	PF08337	Plexin cytoplasmic region
HMMSmart	IPR001627	37	453	SM00630	Semaphorin/CD100 antigen
	IPR003659	471	522	SM00423	Plexin/semaphorin/integrin
	IPR003659	617	670	SM00423	Plexin/semaphorin/integrin
	IPR003659	762	804	SM00423	Plexin/semaphorin/integrin
	IPR002909	805	896	SM00429	Cell surface receptor IPT/TIG
	IPR002909	897	983	SM00429	Cell surface receptor IPT/TIG
	IPR002909	985	1095	SM00429	Cell surface receptor IPT/TIG
ProfileScan	IPR001627	11	469	PS51004	Semaphorin/CD100 antigen

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	AMALQLWALTLLGLLGAGASLR	24	PRIMARY	22
	2	1199	VPLSLILPLVIVPMVVVIAVSV	1220	PRIMARY	22

RT-PCR

Experimental conditions

Chromosome No. 22
Experimental conditions

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