Gene/Protein Characteristic Table for KIAA0400
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Product ID ORK01973
Accession No AB007860
Description ArfGAP with SH3 domain, ankyrin repeat and PH domain 2, transcript variant 1
Clone name hg01091
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5711 bp)
Predicted protein sequence (1022 aa)
Flexi ORF Clone FXC01973
Source Human adult brain
Rouge ID mKIAA0400 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5711 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Features of the protein sequence
Description

Length: 1022 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O43150 0 100.0 Arf-GAP with SH...
Homo sapiens
XP_001088227 0 97.6 similar to deve...
Macaca mulatta
XP_532867 0 95.2 similar to deve...
Canis lupus fam...
XP_515290 0 97.3 development- an...
Pan troglodytes
NP_001128664 0 94.0 ArfGAP with SH3...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB033075 7.6e-52 53.0 KIAA1249
D26069 1.1e-06 28.4 KIAA0041
AB051503 8e-05 26.5 KIAA1716
AB075855 0.00036 28.0 KIAA1975
AB029022 0.0004 27.2 KIAA1099
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001452 963 1017 PD000066 Src homology-3
FPrintScan IPR001164 449 468 PR00405 Arf GTPase activating protein
IPR001164 468 485 PR00405 Arf GTPase activating protein
IPR001164 489 510 PR00405 Arf GTPase activating protein
IPR001452 977 992 PR00452 Src homology-3
IPR001452 994 1003 PR00452 Src homology-3
IPR001452 1008 1020 PR00452 Src homology-3
HMMPfam IPR001849 322 413 PF00169 Pleckstrin-like
IPR001164 437 559 PF01412 Arf GTPase activating protein
IPR002110 600 635 PF00023 Ankyrin
IPR002110 636 668 PF00023 Ankyrin
IPR002110 669 689 PF00023 Ankyrin
IPR001452 963 1017 PF00018 Src homology-3
HMMSmart IPR001849 322 415 SM00233 Pleckstrin-like
IPR001164 437 557 SM00105 Arf GTPase activating protein
IPR002110 600 632 SM00248 Ankyrin
IPR002110 636 665 SM00248 Ankyrin
IPR002110 669 699 SM00248 Ankyrin
IPR001452 963 1021 SM00326 Src homology-3
ProfileScan IPR001849 321 413 PS50003 Pleckstrin-like
IPR001164 437 559 PS50115 Arf GTPase activating protein
IPR002110 600 691 PS50297 Ankyrin
IPR002110 636 668 PS50088 Ankyrin
IPR001452 960 1022 PS50002 Src homology-3
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f GCTGCAACCAAAGTAATCAGG
Primer_r GTGTGATCTGTTAAGGGGTGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f GCTGCAACCAAAGTAATCAGG
Primer_r GTGTGATCTGTTAAGGGGTGG
PCR product length 100 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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