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Order Kazusa clone(s) from : |
| Product ID | ORK01666 |
|---|---|
| Accession No | AB007867 |
| Description | plexin B1, transcript variant 1 |
| Clone name | hg01339 |
| Vector information | |
| cDNA sequence | DNA sequence (7308 bp) Predicted protein sequence (2143 aa) |
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HaloTag ORF Clone |
FHC01666
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| Flexi ORF Clone | FXC01666 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0407
by Kazusa Mouse cDNA Project
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Length: 7308 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 2143 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001627 | 43 | 470 | PF01403 | Semaphorin/CD100 antigen |
| IPR002165 | 489 | 542 | PF01437 | Plexin | |
| IPR002165 | 1029 | 1076 | PF01437 | Plexin | |
| IPR002909 | 1078 | 1167 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1170 | 1256 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1260 | 1382 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR013548 | 1569 | 2110 | PF08337 | Plexin cytoplasmic region | |
| HMMSmart | IPR001627 | 43 | 471 | SM00630 | Semaphorin/CD100 antigen |
| IPR003659 | 489 | 542 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 636 | 686 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 1029 | 1076 | SM00423 | Plexin/semaphorin/integrin | |
| IPR002909 | 1077 | 1168 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1169 | 1257 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1259 | 1383 | SM00429 | Cell surface receptor IPT/TIG | |
| ProfileScan | IPR001627 | 17 | 487 | PS51004 | Semaphorin/CD100 antigen |
| ScanRegExp | IPR003006 | 1028 | 1034 | PS00290 | Immunoglobulin/major histocompatibility complex motif |
| IPR002345 | 1829 | 1841 | PS00213 | Lipocalin |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 7 | VTMPALGPALLQALWAGWVLTLQ | 29 | SECONDARY | 23 | 2 | 1494 | PVAAQVGLGVGTSLLALGVIIIV | 1516 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | AAAAGCTGGGTCAATGGAGTC |
|---|---|
| Primer_r | TTGCTGCTGCCACATCCTAGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 3
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AAAAGCTGGGTCAATGGAGTC |
| Primer_r | TTGCTGCTGCCACATCCTAGG |
| PCR product length | 176 bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |