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Order Kazusa clone(s) from : |
| Product ID | ORK04208 |
|---|---|
| Accession No | AB011138 |
| Description | ATPase, class V, type 10A |
| Clone name | hh01910 |
| Vector information | |
| cDNA sequence | DNA sequence (5562 bp) Predicted protein sequence (1163 aa) |
| Source | Human adult brain |
| Rouge ID |
mKIAA0566
by Kazusa Mouse cDNA Project
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Length: 5562 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | Warning |
Length: 1163 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR001757 | 89 | 103 | PR00119 | ATPase |
| IPR001757 | 693 | 712 | PR00119 | ATPase | |
| HMMPfam | IPR013200 | 687 | 710 | PF08282 | HAD superfamily hydrolase-like |
| HMMTigr | IPR006539 | 3 | 973 | TIGR01652 | Phospholipid-translocating P-type ATPase |
| IPR001757 | 521 | 570 | TIGR01494 | ATPase | |
| IPR001757 | 662 | 773 | TIGR01494 | ATPase | |
| ScanRegExp | IPR001757 | 91 | 97 | PS00154 | ATPase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 24 | YSFLTMIIVLQVLIPISLYVSIE | 46 | PRIMARY | 23 | 2 | 772 | FFCGFSASTMIDQWYLIFFNLLF | 794 | PRIMARY | 23 | 3 | 836 | WFNMADAAFQSLVCFSIPYLAYY | 858 | SECONDARY | 23 | 4 | 865 | FTWGTPIVTIALLTFLLHLGIET | 887 | PRIMARY | 23 | 5 | 895 | WITCGFSVLLFFTVALIYNASCA | 917 | SECONDARY | 23 | 6 | 930 | QALLGDPVFYLTCLMTPVAALLP | 952 | SECONDARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | TTTCCATAATACTCCCCATCC |
|---|---|
| Primer_r | CTCTTGGGAAACGGAATGACC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 15
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TTTCCATAATACTCCCCATCC |
| Primer_r | CTCTTGGGAAACGGAATGACC |
| PCR product length | 124 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |