KIAA0715

Product ID	ORK01674
Accession No	AB018258
Description	ATPase, class V, type 10B
Clone name	pf08610
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7380 bp) Predicted protein sequence (1498 aa)
HaloTag ORF Clone	FHC01674
Flexi ORF Clone	FXC01674
Source	Human brain (hippocampus)
Rouge ID	mKIAA0715 by Kazusa Mouse cDNA Project
Note	We replaced hj02886, former representative clones for KIAA0715 with pf08610. (1999/6/16)

Length: 7380 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2320 bp
Genome contig ID	gi51511721r_159822718
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GTCATCCTTTATTAAAAATAAACATGGTTTTCCAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTGCCTGCCAACCTAAGTTCACATTATTCTTCTTTTATATCATTATCA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	r	159922718	160211624	26	99.9	Perfect prediction

Length: 1498 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10372	100.0	phospholipid-tr...	synthetic construct
O94823	99.9	Probable phosph...	Homo sapiens
EAW61546	94.5	hCG1979529, iso...	Homo sapiens
XP_001086695	88.6	ATPase, Class V...	Macaca mulatta
XP_546266	85.5	similar to Pote...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011138	7.3e-128	50.3	KIAA0566
AB040920	9e-122	57.7	KIAA1487
AB075819	4.8e-59	31.4	KIAA1939
AB032963	4.6e-58	31.9	KIAA1137
AB028944	5.8e-54	32.1	KIAA1021

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	468	482	PR00119	ATPase
FPrintScan	IPR001757	1090	1109	PR00119	ATPase
HMMPfam	IPR013200	1086	1107	PF08282	HAD superfamily hydrolase-like
HMMTigr	IPR006539	103	1370	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	400	487	TIGR01494	ATPase
	IPR001757	919	969	TIGR01494	ATPase
	IPR001757	1059	1170	TIGR01494	ATPase
ScanRegExp	IPR001757	470	476	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	128	HRWANLYFLFLVILNWMPSMEVF	150	SECONDARY	23
	2	153	EITMLPLAIVLFVIMIKDGMEDF	175	PRIMARY	23
	3	351	NIDIFFCIGILILMCLIGAVGHS	373	PRIMARY	23
	4	394	FLPSALGGFYMFLTMIILLQVLI	416	PRIMARY	23
	5	419	SLYVSIELVKLGQVFFLSNDLD	440	SECONDARY	22
	6	1159	CYVNLLFWYQFFCGFSSSTMID	1180	SECONDARY	22
	7	1230	STFWISMVDAFYQSLICFFIPYL	1252	SECONDARY	23
	8	1297	GSFLMYFLVSLLYNATCVICNSP	1319	PRIMARY	23
	9	1335	FYLVCFLTPVVALLPRYFFLSLQ	1357	SECONDARY	23

Primer_f	AGAACCTCCTCAGCAAAGATG
Primer_r	ATGGCAGAGGCAGTGAAACAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AGAACCTCCTCAGCAAAGATG
Primer_r	ATGGCAGAGGCAGTGAAACAG
PCR product length	156 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles