|
Order Kazusa clone(s) from : |
| Product ID | ORK00786 |
|---|---|
| Accession No | AB033086 |
| Description | neuroligin 4, X-linked, transcript variant 1 |
| Clone name | hh15752 |
| Vector information | |
| cDNA sequence | DNA sequence (5454 bp) Predicted protein sequence (817 aa) |
|
HaloTag ORF Clone |
FHC00786
|
| Flexi ORF Clone | FXC00786 |
| Source | Human adult brain |
Length: 5454 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 2539 bp |
|---|---|
| Genome contig ID | gi89161218r_5718319 |
| PolyA signal sequence (AATATA,-18) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Length: 817 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR000460 | 437 | 451 | PR01090 | Neuroligin |
| IPR000460 | 540 | 561 | PR01090 | Neuroligin | |
| IPR000460 | 570 | 588 | PR01090 | Neuroligin | |
| IPR000460 | 670 | 699 | PR01090 | Neuroligin | |
| HMMPfam | IPR002018 | 26 | 591 | PF00135 | Carboxylesterase |
| ScanRegExp | IPR002018 | 145 | 155 | PS00941 | Carboxylesterase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 13 | LLFTPVCVMLNSNVLLWLTALAI | 35 | PRIMARY | 23 | 2 | 676 | LSVTIAVGASLLFLNILAFAAL | 697 | PRIMARY | 22 |
|---|
RT-PCR-ELISA
|
Experimental conditions| Primer_f | AGTAAATACGGCTCTGTGTTC |
|---|---|
| Primer_r | GATAAATAAGGGACCGAGTGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AGTAAATACGGCTCTGTGTTC |
| Primer_r | GATAAATAAGGGACCGAGTGC |
| PCR product length | 135 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |