FLJ00127

Product ID	ORK04327
Accession No	AK074056
Clone name	sh01274
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5859 bp) Predicted protein sequence (296 aa)
Source	Human spleen
Rouge ID	mFLJ00127 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 296 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84882	2.8e-133	100.0	FLJ00127 protei...	Homo sapiens
EAW95474	1.1e-112	99.6	chromosome 16 o...	Homo sapiens
Q8N4N3	4.3e-104	99.2	Kelch-like prot...	Homo sapiens
XP_001112834	5e-104	98.7	similar to diab...	Macaca mulatta
XP_001151795	5e-104	98.7	similar to Chro...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037775	4.3e-41	44.9	KIAA1354
AB037730	6.9e-41	44.9	KIAA1309
AB037805	2.1e-31	36.7	KIAA1384
AB067487	5.9e-25	36.9	KIAA1900
AB040923	1.3e-18	30.3	KIAA1490

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006651	225	238	PR00501	Kelch motif
FPrintScan	IPR006651	244	258	PR00501	Kelch motif
HMMPfam	NULL	66	111	PF07646	NULL
	IPR006652	66	111	PF01344	Kelch repeat
	IPR006652	113	160	PF01344	Kelch repeat
	NULL	162	213	PF07646	NULL
	IPR006652	162	213	PF01344	Kelch repeat
	NULL	215	262	PF07646	NULL
	IPR006652	215	262	PF01344	Kelch repeat

Primer_f
Primer_r
PCR conditions	test