FLJ00139

Product ID	ORK05991
Accession No	AK074068
Clone name	sh03241
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5250 bp) Predicted protein sequence (585 aa)
Source	Human spleen
Rouge ID	mFLJ00139 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 585 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84894	1.2e-157	100.0	FLJ00139 protei...	Homo sapiens
Q8IY33	5.3e-153	100.0	MICAL-like prot...	Homo sapiens
ACE86775	8.7e-153	99.8	MICAL-like 2 pr...	synthetic construct
DAA01346	1.1e-152	99.8	TPA_exp: MICAL-...	Homo sapiens
ACE87461	1.7e-152	99.6	MICAL-like 2 pr...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051455	1.4e-06	33.1	KIAA1668
AK160384	3.2e-06	26.4	FLJ00407
AB018293	1.7e-05	26.9	KIAA0750
AB037785	0.00019	28.4	KIAA1364
AK024451	0.0007	31.3	FLJ00043

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001781	201	254	PD000094	Zn-binding protein
HMMPfam	IPR001715	16	120	PF00307	Calponin-like actin-binding
HMMPfam	IPR001781	201	259	PF00412	Zn-binding protein
HMMSmart	IPR001715	16	115	SM00033	Calponin-like actin-binding
HMMSmart	IPR001781	200	254	SM00132	Zn-binding protein
ProfileScan	IPR001715	14	117	PS50021	Calponin-like actin-binding
ProfileScan	IPR001781	199	261	PS50023	Zn-binding protein
ScanRegExp	IPR001781	201	235	PS00478	Zn-binding protein

Primer_f
Primer_r
PCR conditions	test