FLJ00148

Product ID	ORK05096
Accession No	AK074077
Clone name	sh05404
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5334 bp) Predicted protein sequence (572 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 572 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84903	100.0	FLJ00148 protei...	Homo sapiens
EAW87286	100.0	sidekick homolo...	Homo sapiens
NP_689957	100.0	sidekick 1 isof...	Homo sapiens
AAP75619	100.0	sidekick-like p...	Homo sapiens
Q7Z5N4	100.0	Protein sidekic...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK074083	1.5e-191	99.8	FLJ00154
AB040947	1.1e-59	58.1	KIAA1514
AB002341	3.8e-15	30.7	KIAA0343
AB032958	5.6e-15	30.3	KIAA1132
AB040929	4.4e-12	33.5	KIAA1496

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003962	75	84	PR00014	Fibronectin
	IPR003962	90	100	PR00014	Fibronectin
	IPR003962	315	333	PR00014	Fibronectin
	IPR003962	333	347	PR00014	Fibronectin
HMMPfam	IPR003961	60	149	PF00041	Fibronectin
	IPR003961	161	250	PF00041	Fibronectin
	IPR003961	262	349	PF00041	Fibronectin
HMMSmart	IPR003961	61	146	SM00060	Fibronectin
	IPR003961	161	248	SM00060	Fibronectin
	IPR003961	262	346	SM00060	Fibronectin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	368	WFLLVMALSSLIVILLVVFALVL	390	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test