FLJ00371

Product ID	ORK05178
Accession No	AK090450
Clone name	sh06716
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5657 bp) Predicted protein sequence (625 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 625 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC03431	5.3e-179	100.0	FLJ00371 protei...	Homo sapiens
BAB84940	7.6e-176	99.8	FLJ00185 protei...	Homo sapiens
EAW88037	8.1e-170	99.7	ral guanine nuc...	Homo sapiens
AAQ13414	8.2e-170	99.7	Ral guanine nuc...	Homo sapiens
BAH13489	8.4e-170	99.7	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK074114	6.7e-95	99.8	FLJ00185
AB037729	3e-79	98.1	KIAA1308
AB023176	5.8e-31	51.5	KIAA0959
AK074082	7.2e-20	42.4	FLJ00153

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001895	172	386	PF00617	Guanine-nucleotide dissociation stimulator CDC25
HMMSmart	IPR001895	171	438	SM00147	Guanine-nucleotide dissociation stimulator CDC25
ProfileScan	IPR000694	42	157	PS50099	Proline-rich region
	IPR001895	174	436	PS50009	Guanine-nucleotide dissociation stimulator CDC25
	NULL	464	576	PS50324	NULL
ScanRegExp	IPR001895	353	383	PS00720	Guanine-nucleotide dissociation stimulator CDC25

Primer_f
Primer_r
PCR conditions	test