HUGE

Gene/Protein Characteristic Table for KIAA0231

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00037
Accession No. :	D86984
Description :	Leucine-rich repeat-containing protein 8B.
HUGO Gene Name :	leucine rich repeat containing 8 family, member B (LRRC8B)
Clone Name :	fk16230 [Vector Info]
Flexi ORF Clone :	pF1KA0231
Source :	Human fetal brain
Note :	We replaced ha02410, former representative clones for KIAA0231 with fk16230. (2005/08/06)

Features of the cloned DNA sequence	Description

Length: 3615 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 333 bp

Genome contig ID gi89161185f_89687489
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CACCAGCCTGGGTGACAGAGCAAGACTCTTATCTC
Flanking genome sequence
(144037 - 144086)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAATGCTCCAGGGCTTTAAATGAGAAGTAAAATTTTC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 f 89787489 89831524 6 99.7 Perfect prediction

Features of the protein sequence	Description

Length: 823 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q6P9F7	100.0	Leucine-rich re...	Homo sapiens
AAH60782	99.9	LRRC8B protein ...	Homo sapiens
XP_001150203	99.8	T-cell activati...	Pan troglodytes
AAQ88479	99.8	ITLT6413 [Homo ...	Homo sapiens
XP_001494263	97.0	leucine rich re...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037858	5.4e-172	59.0	KIAA1437
AB020669	4.8e-18	27.4	KIAA0862
AB033051	5.2e-10	29.2	KIAA1225
D63481	3e-09	30.8	KIAA0147
AB046800	4.4e-07	29.8	KIAA1580

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001611 630 643 PR00019 Leucine-rich repeat

IPR001611 675 688 PR00019 Leucine-rich repeat

HMMPfam IPR001611 531 557 PF00560 Leucine-rich repeat

IPR001611 606 627 PF00560 Leucine-rich repeat

IPR001611 629 652 PF00560 Leucine-rich repeat

IPR001611 654 675 PF00560 Leucine-rich repeat

IPR001611 677 698 PF00560 Leucine-rich repeat

IPR001611 700 721 PF00560 Leucine-rich repeat

IPR001611 769 790 PF00560 Leucine-rich repeat

HMMSmart IPR003591 529 556 SM00369 Leucine-rich repeat

IPR003591 604 627 SM00369 Leucine-rich repeat

IPR003591 629 650 SM00369 Leucine-rich repeat

IPR003591 652 674 SM00369 Leucine-rich repeat

IPR003591 675 698 SM00369 Leucine-rich repeat

IPR003591 699 720 SM00369 Leucine-rich repeat

IPR003591 721 744 SM00369 Leucine-rich repeat

IPR003591 767 790 SM00369 Leucine-rich repeat

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	630	643	PR00019	Leucine-rich repeat
IPR001611	675	688	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	531	557	PF00560	Leucine-rich repeat
IPR001611	606	627	PF00560	Leucine-rich repeat
IPR001611	629	652	PF00560	Leucine-rich repeat
IPR001611	654	675	PF00560	Leucine-rich repeat
IPR001611	677	698	PF00560	Leucine-rich repeat
IPR001611	700	721	PF00560	Leucine-rich repeat
IPR001611	769	790	PF00560	Leucine-rich repeat
HMMSmart	IPR003591	529	556	SM00369	Leucine-rich repeat
IPR003591	604	627	SM00369	Leucine-rich repeat
IPR003591	629	650	SM00369	Leucine-rich repeat
IPR003591	652	674	SM00369	Leucine-rich repeat
IPR003591	675	698	SM00369	Leucine-rich repeat
IPR003591	699	720	SM00369	Leucine-rich repeat
IPR003591	721	744	SM00369	Leucine-rich repeat
IPR003591	767	790	SM00369	Leucine-rich repeat

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 5 LFNFFHCISVLLQGKVMITLTEL 27 SECONDARY 23

2 283 IVKVILFVLIITYVPYFLTHITL 305 PRIMARY 23

3 325 VYSLAEIFKVLASFYVILVILYG 347 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	LFNFFHCISVLLQGKVMITLTEL	27	SECONDARY	23
2	283	IVKVILFVLIITYVPYFLTHITL	305	PRIMARY	23
3	325	VYSLAEIFKVLASFYVILVILYG	347	PRIMARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

Experimental conditions

Panel name	:
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	333 bp
Genome contig ID	gi89161185f_89687489
PolyA signal sequence (None)	+--------+--------+----*----+---- CACCAGCCTGGGTGACAGAGCAAGACTCTTATCTC
Flanking genome sequence (144037 - 144086)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAATGCTCCAGGGCTTTAAATGAGAAGTAAAATTTTC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	f	89787489	89831524	6	99.7	Perfect prediction

Gene/Protein Characteristic Table for KIAA0231

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information