HUGE

Gene/Protein Characteristic Table for KIAA1437

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00834
Accession No. :	AB037858
Description :	Leucine-rich repeat-containing protein 8A.
HUGO Gene Name :	leucine rich repeat containing 8 family, member A (LRRC8A)
Clone Name :	hk07206 [Vector Info]
Flexi ORF Clone :	pF1KSDA1437
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4161 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1665 bp

Genome contig ID gi89161216f_130584296
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
GACTCGGTCACAGTATCAAATAAAATCTATAACAG
Flanking genome sequence
(135842 - 135891)
----+----*----+----*----+----*----+----*----+----*
AAAGTGGCCTTTGGAGGTCTTTGTGTCATGGGAGGACGCTTCCCTCACTT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 f 130684296 130720136 3 99.0 Perfect prediction

Features of the protein sequence	Description

Length: 811 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q8IWT6	100.0	Leucine-rich re...	Homo sapiens
ABY84167	99.9	leucine-rich re...	Callithrix jacchus
BAF82531	99.8	unnamed protein...	Homo sapiens
XP_001500311	99.4	similar to Leuc...	Equus caballus
AAI23433	99.0	Leucine rich re...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D86984	2.3e-175	59.0	KIAA0231
D63481	4e-14	30.6	KIAA0147
AB020669	3.8e-13	28.9	KIAA0862
AB033051	1.3e-11	28.1	KIAA1225
AB023233	1.9e-09	30.4	KIAA1016

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001611 688 701 PR00019 Leucine-rich repeat

IPR001611 754 767 PR00019 Leucine-rich repeat

HMMPfam IPR001611 593 614 PF00560 Leucine-rich repeat

IPR001611 616 636 PF00560 Leucine-rich repeat

IPR001611 641 662 PF00560 Leucine-rich repeat

IPR001611 664 685 PF00560 Leucine-rich repeat

IPR001611 687 708 PF00560 Leucine-rich repeat

IPR001611 710 731 PF00560 Leucine-rich repeat

IPR001611 733 754 PF00560 Leucine-rich repeat

IPR001611 756 777 PF00560 Leucine-rich repeat

HMMSmart IPR003591 591 614 SM00369 Leucine-rich repeat

NULL 614 635 SM00365 NULL

IPR003591 615 637 SM00369 Leucine-rich repeat

IPR003591 639 661 SM00369 Leucine-rich repeat

NULL 662 693 SM00365 NULL

IPR003591 662 685 SM00369 Leucine-rich repeat

IPR003591 686 707 SM00369 Leucine-rich repeat

IPR003591 708 731 SM00369 Leucine-rich repeat

IPR003591 732 752 SM00369 Leucine-rich repeat

NULL 754 774 SM00365 NULL

IPR003591 754 777 SM00369 Leucine-rich repeat

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	688	701	PR00019	Leucine-rich repeat
IPR001611	754	767	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	593	614	PF00560	Leucine-rich repeat
IPR001611	616	636	PF00560	Leucine-rich repeat
IPR001611	641	662	PF00560	Leucine-rich repeat
IPR001611	664	685	PF00560	Leucine-rich repeat
IPR001611	687	708	PF00560	Leucine-rich repeat
IPR001611	710	731	PF00560	Leucine-rich repeat
IPR001611	733	754	PF00560	Leucine-rich repeat
IPR001611	756	777	PF00560	Leucine-rich repeat
HMMSmart	IPR003591	591	614	SM00369	Leucine-rich repeat
NULL	614	635	SM00365	NULL
IPR003591	615	637	SM00369	Leucine-rich repeat
IPR003591	639	661	SM00369	Leucine-rich repeat
NULL	662	693	SM00365	NULL
IPR003591	662	685	SM00369	Leucine-rich repeat
IPR003591	686	707	SM00369	Leucine-rich repeat
IPR003591	708	731	SM00369	Leucine-rich repeat
IPR003591	732	752	SM00369	Leucine-rich repeat
NULL	754	774	SM00365	NULL
IPR003591	754	777	SM00369	Leucine-rich repeat

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 26 DVFTDYISIVMLMIAVFGGTLQV 48 SECONDARY 23

2 266 MRQTIIKVIKFILIICYTVYYVH 288 PRIMARY 23

3 319 FKILASFYISLVIFYGLICMYTL 341 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	26	DVFTDYISIVMLMIAVFGGTLQV	48	SECONDARY	23
2	266	MRQTIIKVIKFILIICYTVYYVH	288	PRIMARY	23
3	319	FKILASFYISLVIFYGLICMYTL	341	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCCCCGCCTTAGATCAATCAC

Primer_r
: GGTTGTCAGAGGCTAGTGTGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1665 bp
Genome contig ID	gi89161216f_130584296
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- GACTCGGTCACAGTATCAAATAAAATCTATAACAG
Flanking genome sequence (135842 - 135891)	----+--------+--------+--------+--------+----* AAAGTGGCCTTTGGAGGTCTTTGTGTCATGGGAGGACGCTTCCCTCACTT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	f	130684296	130720136	3	99.0	Perfect prediction

Primer_f	: TCCCCGCCTTAGATCAATCAC
Primer_r	: GGTTGTCAGAGGCTAGTGTGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1437

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information