HUGE

Gene/Protein Characteristic Table for KIAA0758

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00625
Accession No. :	AB018301
Description :	Probable G-protein coupled receptor 116 precursor.
HUGO Gene Name :	G protein-coupled receptor 116 (GPR116)
Clone Name :	hh15259 [Vector Info]
Flexi ORF Clone :	pF1KSDA0758
Source :	Human adult brain
Note :	We replaced hk04615, former representative clones for KIAA0758 with hh15259. (2005/08/06)

Features of the cloned DNA sequence	Description

Length: 5655 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1387 bp

Genome contig ID gi89161210r_46828301
PolyA signal sequence
(ATTAAA,-20)
+----*----+----*----+----*----+----
TTTGGTGTGTATTTCATTAAAAAATATAAATATTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATGAAAATTGCACGCATATTAGAGTTAACCATGTACTATTGATACAGCAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 6 r 46928301 46997611 21 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 1370 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001140861	99.3	G-protein coupl...	Pan troglodytes
EAX04309	99.9	G protein-coupl...	Homo sapiens
Q8IZF2	100.0	Probable G-prot...	Homo sapiens
EAX04307	99.9	G protein-coupl...	Homo sapiens
AAS21061	99.9	G protein coupl...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	5.3e-09	25.6	KIAA0768
AB020628	3.2e-08	23.7	KIAA0821
AB018329	7.2e-08	24.6	KIAA0786
D87469	9e-06	27.1	KIAA0279
AB011536	0.00034	20.9	KIAA0812

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR008078 84 102 PR01695 Ig-hepta receptor

IPR008078 163 188 PR01695 Ig-hepta receptor

IPR008078 400 417 PR01695 Ig-hepta receptor

IPR008078 422 448 PR01695 Ig-hepta receptor

IPR008078 451 475 PR01695 Ig-hepta receptor

IPR008078 690 711 PR01695 Ig-hepta receptor

IPR008078 724 737 PR01695 Ig-hepta receptor

IPR000832 1038 1062 PR00249 GPCR

IPR008078 1062 1074 PR01695 Ig-hepta receptor

IPR000832 1112 1135 PR00249 GPCR

IPR008078 1136 1152 PR01695 Ig-hepta receptor

IPR008078 1178 1195 PR01695 Ig-hepta receptor

IPR000832 1197 1222 PR00249 GPCR

IPR008078 1223 1240 PR01695 Ig-hepta receptor

IPR000832 1241 1261 PR00249 GPCR

IPR000832 1272 1293 PR00249 GPCR

IPR008078 1296 1322 PR01695 Ig-hepta receptor

IPR008078 1324 1348 PR01695 Ig-hepta receptor

HMMPfam IPR000082 191 290 PF01390 SEA

IPR013151 310 376 PF00047 Immunoglobulin

IPR013151 509 571 PF00047 Immunoglobulin

IPR000203 974 1021 PF01825 GPS

IPR000832 1080 1297 PF00002 GPCR

HMMSmart IPR003599 302 392 SM00409 Immunoglobulin subtype

IPR003598 308 381 SM00408 Immunoglobulin subtype 2

IPR003599 501 587 SM00409 Immunoglobulin subtype

IPR003598 507 576 SM00408 Immunoglobulin subtype 2

IPR000203 974 1026 SM00303 GPS

ProfileScan IPR007110 291 376 PS50835 Immunoglobulin-like

IPR007110 393 490 PS50835 Immunoglobulin-like

IPR007110 495 585 PS50835 Immunoglobulin-like

IPR000203 975 1026 PS50221 GPS

IPR000832 1036 1294 PS50261 GPCR

ScanRegExp IPR013032 144 157 PS01186 EGF-like region

IPR000832 1282 1297 PS00650 GPCR

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008078	84	102	PR01695	Ig-hepta receptor
IPR008078	163	188	PR01695	Ig-hepta receptor
IPR008078	400	417	PR01695	Ig-hepta receptor
IPR008078	422	448	PR01695	Ig-hepta receptor
IPR008078	451	475	PR01695	Ig-hepta receptor
IPR008078	690	711	PR01695	Ig-hepta receptor
IPR008078	724	737	PR01695	Ig-hepta receptor
IPR000832	1038	1062	PR00249	GPCR
IPR008078	1062	1074	PR01695	Ig-hepta receptor
IPR000832	1112	1135	PR00249	GPCR
IPR008078	1136	1152	PR01695	Ig-hepta receptor
IPR008078	1178	1195	PR01695	Ig-hepta receptor
IPR000832	1197	1222	PR00249	GPCR
IPR008078	1223	1240	PR01695	Ig-hepta receptor
IPR000832	1241	1261	PR00249	GPCR
IPR000832	1272	1293	PR00249	GPCR
IPR008078	1296	1322	PR01695	Ig-hepta receptor
IPR008078	1324	1348	PR01695	Ig-hepta receptor
HMMPfam	IPR000082	191	290	PF01390	SEA
IPR013151	310	376	PF00047	Immunoglobulin
IPR013151	509	571	PF00047	Immunoglobulin
IPR000203	974	1021	PF01825	GPS
IPR000832	1080	1297	PF00002	GPCR
HMMSmart	IPR003599	302	392	SM00409	Immunoglobulin subtype
IPR003598	308	381	SM00408	Immunoglobulin subtype 2
IPR003599	501	587	SM00409	Immunoglobulin subtype
IPR003598	507	576	SM00408	Immunoglobulin subtype 2
IPR000203	974	1026	SM00303	GPS
ProfileScan	IPR007110	291	376	PS50835	Immunoglobulin-like
IPR007110	393	490	PS50835	Immunoglobulin-like
IPR007110	495	585	PS50835	Immunoglobulin-like
IPR000203	975	1026	PS50221	GPS
IPR000832	1036	1294	PS50261	GPCR
ScanRegExp	IPR013032	144	157	PS01186	EGF-like region
IPR000832	1282	1297	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 31 TTLCLMFIVIYSSKAALNWNYES 53 SECONDARY 23

2 1036 LDIISYVGVGFSILSLAACLVVE 1058 PRIMARY 23

3 1076 TCIVNIAASLLVANTWFIVVAAI 1098 SECONDARY 23

4 1114 TFFIHFFYLSVFFWMLTLGLMLF 1136 PRIMARY 23

5 1152 KAIAFCLGYGCPLAISVITLGAT 1174 SECONDARY 23

6 1198 AFAIPALIIVVVNITITIVVITK 1220 PRIMARY 23

7 1239 FQISKSIGVLTPLLGLTWGFGLT 1261 SECONDARY 23

8 1272 HIIFAILNVFQGLFILLFGCLWD 1294 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	31	TTLCLMFIVIYSSKAALNWNYES	53	SECONDARY	23
2	1036	LDIISYVGVGFSILSLAACLVVE	1058	PRIMARY	23
3	1076	TCIVNIAASLLVANTWFIVVAAI	1098	SECONDARY	23
4	1114	TFFIHFFYLSVFFWMLTLGLMLF	1136	PRIMARY	23
5	1152	KAIAFCLGYGCPLAISVITLGAT	1174	SECONDARY	23
6	1198	AFAIPALIIVVVNITITIVVITK	1220	PRIMARY	23
7	1239	FQISKSIGVLTPLLGLTWGFGLT	1261	SECONDARY	23
8	1272	HIIFAILNVFQGLFILLFGCLWD	1294	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ATTGTGGATACTGGCCCTTGG

Primer_r
: CATTAGGAGCACAGAACAACC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 6

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: ATTGTGGATACTGGCCCTTGG
Primer_r	: CATTAGGAGCACAGAACAACC
PCR product length	: 178 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1387 bp
Genome contig ID	gi89161210r_46828301
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- TTTGGTGTGTATTTCATTAAAAAATATAAATATTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATGAAAATTGCACGCATATTAGAGTTAACCATGTACTATTGATACAGCAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	6	r	46928301	46997611	21	99.4	Perfect prediction

Gene/Protein Characteristic Table for KIAA0758

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information