HUGE

Gene/Protein Characteristic Table for KIAA0999

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK06551
Accession No. :	AB023216
Description :	Serine/threonine-protein kinase QSK.
HUGO Gene Name :
Clone Name :	fj10213 [Vector Info]
Source :	Human fetal brain
Note :	We replaced hk08707, former representative clones for KIAA0999 with fj10213. (2001/5/29)

Features of the cloned DNA sequence	Description

Length: 4460 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Features of the protein sequence	Description

Length: 1371 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_582999	0	93.0	similar to Seri...	Bos taurus
XP_001500846	0	95.4	similar to Seri...	Equus caballus
EDL25676	1e-162	89.7	cDNA sequence B...	Mus musculus
AAH82313	8.8e-155	87.3	BC033915 protei...	Mus musculus
NP_081774	2.7e-152	87.8	serine/threonin...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018324	6.3e-40	36.7	KIAA0781
AB058763	7.8e-31	52.2	KIAA1860
D43636	1.4e-21	38.0	KIAA0096
AB040910	2.7e-21	51.7	KIAA1477
AB011109	5.9e-21	37.5	KIAA0537

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

BlastProDom IPR000719 116 367 PD000001 Protein kinase

HMMPfam IPR000719 116 367 PF00069 Protein kinase

HMMSmart IPR001245 116 367 SM00219 Tyrosine protein kinase

IPR002290 116 367 SM00220 Serine/threonine protein kinase

ProfileScan IPR000719 116 367 PS50011 Protein kinase

IPR000449 394 434 PS50030 Ubiquitin-associated/Translation elongation factor EF1B

ScanRegExp IPR000719 122 145 PS00107 Protein kinase

IPR008271 234 246 PS00108 Serine/threonine protein kinase

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000719	116	367	PD000001	Protein kinase
HMMPfam	IPR000719	116	367	PF00069	Protein kinase
HMMSmart	IPR001245	116	367	SM00219	Tyrosine protein kinase
IPR002290	116	367	SM00220	Serine/threonine protein kinase
ProfileScan	IPR000719	116	367	PS50011	Protein kinase
IPR000449	394	434	PS50030	Ubiquitin-associated/Translation elongation factor EF1B
ScanRegExp	IPR000719	122	145	PS00107	Protein kinase
IPR008271	234	246	PS00108	Serine/threonine protein kinase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 7 VGTGARASAVAAVPSVSLLSGAS 29 SECONDARY 23

2 31 SSLAWCGLATGALGAGARGIPEL 53 SECONDARY 23

3 295 DIWSLGVVLYVLVCGALPFDGST 317 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	7	VGTGARASAVAAVPSVSLLSGAS	29	SECONDARY	23
2	31	SSLAWCGLATGALGAGARGIPEL	53	SECONDARY	23
3	295	DIWSLGVVLYVLVCGALPFDGST	317	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCACCAACTACTCACCAGAAG

Primer_r
: CAAGATGGCACGGTTAGTAGG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 11

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TGACTTATCTGCGCTTGTTCC
Primer_r	: CAAGATGGCACGGTTAGTAGG
PCR product length	: 164 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Primer_f	: TCACCAACTACTCACCAGAAG
Primer_r	: CAAGATGGCACGGTTAGTAGG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0999

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information