HUGE

Gene/Protein Characteristic Table for KIAA1070

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Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00172
Accession No. :	AB028993
Description :	neuroligin 1.
HUGO Gene Name :	neuroligin 1 (NLGN1)
Clone Name :	hj05602 [Vector Info]
Flexi ORF Clone :	pF1KA1070
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4960 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1819 bp

Genome contig ID gi89161205f_174685171
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AAAATGTTTATTGCTGTTTTGTCTTTCTTTTTTCT
Flanking genome sequence
(798438 - 798487)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAGAAAAAAAAGAAAAAAAGGAAAAGAAGAACAAAGAAACATGAC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 3 f 174785171 175483607 6 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 826 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH32555	100.0	Neuroligin 1 [H...	Homo sapiens
XP_001166321	99.9	neuroligin 1 is...	Pan troglodytes
XP_001082770	99.3	neuroligin 1 is...	Macaca mulatta
XP_001494442	99.1	neuroligin 1 is...	Equus caballus
XP_545297	98.9	similar to neur...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037787	2.6e-111	63.0	KIAA1366
AB033086	2.1e-110	74.9	KIAA1260
AB023168	2.1e-110	75.0	KIAA0951
AB040913	1.4e-107	71.7	KIAA1480

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000460 455 469 PR01090 Neuroligin

IPR000460 558 579 PR01090 Neuroligin

IPR000460 588 606 PR01090 Neuroligin

IPR000460 673 702 PR01090 Neuroligin

HMMPfam IPR002018 36 609 PF00135 Carboxylesterase

ScanRegExp IPR002018 154 164 PS00941 Carboxylesterase

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000460	455	469	PR01090	Neuroligin
IPR000460	558	579	PR01090	Neuroligin
IPR000460	588	606	PR01090	Neuroligin
IPR000460	673	702	PR01090	Neuroligin
HMMPfam	IPR002018	36	609	PF00135	Carboxylesterase
ScanRegExp	IPR002018	154	164	PS00941	Carboxylesterase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 24 VHRGLGAPLTLCMLGCLLQAGHV 46 SECONDARY 23

2 679 LSVTIAVGASLLFLNILAFAAL 700 PRIMARY 22

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	24	VHRGLGAPLTLCMLGCLLQAGHV	46	SECONDARY	23
2	679	LSVTIAVGASLLFLNILAFAAL	700	PRIMARY	22

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TTCATCCCCCTGTTGTTTACC

Primer_r
: TCAGGATGTGCTCTTTAAGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 3

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TGAAGATGCTGCTCCAATACA
Primer_r	: TATACCTCCGATTTCTTACAG
PCR product length	: 106 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1819 bp
Genome contig ID	gi89161205f_174685171
PolyA signal sequence (None)	+--------+--------+----*----+---- AAAATGTTTATTGCTGTTTTGTCTTTCTTTTTTCT
Flanking genome sequence (798438 - 798487)	----+--------+--------+--------+--------+----* AAAAAAAGAAAAAAAAGAAAAAAAGGAAAAGAAGAACAAAGAAACATGAC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	3	f	174785171	175483607	6	99.2	Perfect prediction

Primer_f	: TTCATCCCCCTGTTGTTTACC
Primer_r	: TCAGGATGTGCTCTTTAAGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1070

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information