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Gene/Protein Characteristic Table for KIAA0080

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Product ID	ORK00398
Accession No	D38522
Description	synaptotagmin XI
Clone name	hh13320
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5974 bp) Predicted protein sequence (486 aa)
HaloTag ORF Clone	FHC00398
Flexi ORF Clone	FXC00398
Source	Human adult brain
Rouge ID	mKIAA0080 by Kazusa Mouse cDNA Project
Note	We replaced ha00491, former representative clones for KIAA0080 with hh13320. (2003/2/13)

Length: 5974 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 486 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001565	216	231	PR00399	Synaptotagmin
	IPR001565	231	244	PR00399	Synaptotagmin
	IPR001565	288	303	PR00399	Synaptotagmin
	IPR001565	308	318	PR00399	Synaptotagmin
	IPR000008	379	391	PR00360	C2 calcium-dependent membrane targeting
	IPR000008	408	421	PR00360	C2 calcium-dependent membrane targeting
	IPR000008	432	440	PR00360	C2 calcium-dependent membrane targeting
HMMPfam	IPR000008	229	317	PF00168	C2 calcium-dependent membrane targeting
	IPR000008	363	452	PF00168	C2 calcium-dependent membrane targeting
HMMSmart	IPR000008	228	332	SM00239	C2 calcium-dependent membrane targeting
	IPR000008	362	478	SM00239	C2 calcium-dependent membrane targeting
ProfileScan	IPR000008	229	317	PS50004	C2 calcium-dependent membrane targeting
	IPR000008	359	452	PS50004	C2 calcium-dependent membrane targeting

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	71	VVAGLIGASVLVVCVSVTVFVWS	93	PRIMARY	23

Northern blot

RT-PCR-ELISA

Experimental conditions

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