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Order Kazusa clone(s) from : |
| Product ID | ORK00420 |
|---|---|
| Accession No | D50922 |
| Description | kelch-like ECH-associated protein 1, transcript variant 2 |
| Clone name | ha01449s1 |
| Vector information | |
| cDNA sequence | DNA sequence (2513 bp) Predicted protein sequence (637 aa) |
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HaloTag ORF Clone |
FHC00420
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| Flexi ORF Clone | FXC00420 |
| Source | Myeloblast cell line (KG-1) |
| Rouge ID |
mKIAA0132
by Kazusa Mouse cDNA Project
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| Note | We replaced ha01449, former representative clones for KIAA0132 with ha01449s1. (2008/12/19) |
Length: 2513 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 526 bp |
|---|---|
| Genome contig ID | gi42406306r_10357802 |
| PolyA signal sequence (AATAAA,-29) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Length: 637 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR006651 | 576 | 589 | PR00501 | Kelch motif |
| IPR006651 | 593 | 607 | PR00501 | Kelch motif | |
| HMMPfam | IPR013069 | 80 | 192 | PF00651 | BTB/POZ |
| IPR011705 | 197 | 299 | PF07707 | BTB/Kelch-associated | |
| IPR006652 | 328 | 372 | PF01344 | Kelch repeat | |
| IPR011498 | 374 | 423 | PF07646 | Kelch | |
| IPR006652 | 374 | 423 | PF01344 | Kelch repeat | |
| IPR011498 | 425 | 470 | PF07646 | Kelch | |
| IPR006652 | 425 | 470 | PF01344 | Kelch repeat | |
| IPR011498 | 472 | 517 | PF07646 | Kelch | |
| IPR006652 | 472 | 517 | PF01344 | Kelch repeat | |
| IPR011498 | 519 | 564 | PF07646 | Kelch | |
| IPR006652 | 519 | 564 | PF01344 | Kelch repeat | |
| IPR011498 | 566 | 611 | PF07646 | Kelch | |
| IPR006652 | 566 | 611 | PF01344 | Kelch repeat | |
| HMMSmart | IPR000210 | 90 | 192 | SM00225 | BTB |
| ProfileScan | IPR000210 | 90 | 162 | PS50097 | BTB |
Experimental conditions| Primer_f | |
|---|---|
| Primer_r | |
| PCR conditions | test |
Chromosome No. 19
Experimental conditions| Panel name | Genebridge 4 |
|---|---|
| Primer_f | TTACGACCCAGATACAGACAC |
| Primer_r | TTCTGCTGGTCAATCTGCTTC |
| PCR product length | 114 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |