|
Order Kazusa clone(s) from : |
| Product ID | ORK00556 |
|---|---|
| Accession No | AB011109 |
| Description | NUAK family, SNF1-like kinase, 1 |
| Clone name | hg03925 |
| Vector information | |
| cDNA sequence | DNA sequence (6828 bp) Predicted protein sequence (698 aa) |
|
HaloTag ORF Clone |
FHC00556
|
| Flexi ORF Clone | FXC00556 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0537
by Kazusa Mouse cDNA Project
|
Length: 6828 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 698 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR000719 | 92 | 342 | PD000001 | Protein kinase |
| HMMPfam | IPR000719 | 92 | 343 | PF00069 | Protein kinase |
| HMMSmart | IPR001245 | 92 | 341 | SM00219 | Tyrosine protein kinase |
| IPR002290 | 92 | 343 | SM00220 | Serine/threonine protein kinase | |
| ProfileScan | IPR000719 | 92 | 343 | PS50011 | Protein kinase |
| ScanRegExp | IPR000719 | 98 | 125 | PS00107 | Protein kinase |
| IPR008271 | 211 | 223 | PS00108 | Serine/threonine protein kinase |
RT-PCR
|
|---|
Experimental conditions| Primer_f | ATGCTAAGTACCCTCTGAATG |
|---|---|
| Primer_r | GCAACAAGCAGTCAGTCGATC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 12
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | ATGCTAAGTACCCTCTGAATG |
| Primer_r | GCAACAAGCAGTCAGTCGATC |
| PCR product length | 150 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |