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Gene/Protein Characteristic Table for KIAA0999

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Product ID	ORK06551
Accession No	AB023216
Description	SIK family kinase 3
Clone name	fj10213
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4460 bp) Predicted protein sequence (1371 aa)
Source	Human fetal brain
Rouge ID	mKIAA0999 by Kazusa Mouse cDNA Project
Note	We replaced hk08707, former representative clones for KIAA0999 with fj10213. (2001/5/29)

Length: 4460 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1371 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000719	116	367	PD000001	Protein kinase
HMMPfam	IPR000719	116	367	PF00069	Protein kinase
HMMSmart	IPR001245	116	367	SM00219	Tyrosine protein kinase
	IPR002290	116	367	SM00220	Serine/threonine protein kinase
ProfileScan	IPR000719	116	367	PS50011	Protein kinase
	IPR000449	394	434	PS50030	Ubiquitin-associated/Translation elongation factor EF1B
ScanRegExp	IPR000719	122	145	PS00107	Protein kinase
	IPR008271	234	246	PS00108	Serine/threonine protein kinase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	7	VGTGARASAVAAVPSVSLLSGAS	29	SECONDARY	23
	2	31	SSLAWCGLATGALGAGARGIPEL	53	SECONDARY	23
	3	295	DIWSLGVVLYVLVCGALPFDGST	317	PRIMARY	23

RT-PCR-ELISA

Experimental conditions

Chromosome No. 11
Experimental conditions

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