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Order Kazusa clone(s) from : |
| Product ID | ORK01983 |
|---|---|
| Accession No | AB011175 |
| Description | TBC1 domain family, member 4, transcript variant 1 |
| Clone name | hg01488b |
| Vector information | |
| cDNA sequence | DNA sequence (5922 bp) Predicted protein sequence (1348 aa) |
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HaloTag ORF Clone |
FHC01983
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| Flexi ORF Clone | FXC01983 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0603
by Kazusa Mouse cDNA Project
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Length: 5922 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1348 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR006020 | 179 | 237 | PF00640 | Phosphotyrosine interaction region |
| IPR000195 | 965 | 1184 | PF00566 | RabGAP/TBC | |
| HMMSmart | IPR006020 | 80 | 240 | SM00462 | Phosphotyrosine interaction region |
| IPR006020 | 246 | 499 | SM00462 | Phosphotyrosine interaction region | |
| IPR000195 | 965 | 1185 | SM00164 | RabGAP/TBC | |
| ProfileScan | IPR006020 | 415 | 487 | PS01179 | Phosphotyrosine interaction region |
| IPR000195 | 968 | 1162 | PS50086 | RabGAP/TBC |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 1145 | QFSLGFVARVFDIIFLQGTEVIF | 1167 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | CTCTTCACTTCCTCAGCCTAC |
|---|---|
| Primer_r | TTACTTTCCTTGCCTATACCC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 13
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CTCTTCACTTCCTCAGCCTAC |
| Primer_r | TTACTTTCCTTGCCTATACCC |
| PCR product length | 74 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |