KIAA1888

Product ID	ORK04007
Accession No	AB067475
Description	ATP-binding cassette, sub-family A (ABC1), member 5
Clone name	fk02242
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3112 bp) Predicted protein sequence (737 aa)
Source	Human fetal brain
Rouge ID	mKIAA1888 by Kazusa Mouse cDNA Project

Length: 3112 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	897 bp
Genome contig ID	gi51511734r_64654387
PolyA signal sequence (AATAAA,-31)	+--------+--------+----*----+---- CTTGAATAAAATATTTTATTGGGTAAAAAAAAAAT
Flanking genome sequence (227358 - 227309)	----+--------+--------+--------+--------+----* AGACAGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	17	r	64754386	64781744	20	99.6	Terminal No-hit

Length: 737 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q8WWZ7	99.9	ATP-binding cas...	Homo sapiens
CAB93535	99.6	ATP-binding cas...	Homo sapiens
AAK30022	99.7	ATP-binding cas...	Homo sapiens
BAB71208	99.3	unnamed protein...	Homo sapiens
XP_511650	98.6	ATP-binding cas...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB020629	1.5e-82	41.9	KIAA0822
AB028985	1.1e-38	28.9	KIAA1062
AB040953	2.1e-05	22.4	KIAA1520

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	528	570	PD000006	ABC transporter related
HMMPfam	IPR003439	421	604	PF00005	ABC transporter related
HMMSmart	IPR003593	420	607	SM00382	AAA+ ATPase
ProfileScan	IPR003439	385	628	PS50893	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	62	VFAAVFNSTMVYSLPILVNIISN	84	SECONDARY	23
	2	113	KIELYFQAALLGIIVTAMPPYFA	135	PRIMARY	23
	3	163	GQAVVDIPLFFIILILMLGSLLA	185	PRIMARY	23
	4	198	LAVVFCLIGYVPSVILFTYIASF	220	PRIMARY	23
	5	232	WSFIYSVAALACIAITEITFFMG	254	PRIMARY	23
	6	262	HYAFCIIIPIYPLLGCLISFIKI	284	PRIMARY	23
	7	302	LSVAVISPYLQCVLWIFLLQ	321	SECONDARY	20

Primer_f	CAGAGACAAGTGAAGATGATG
Primer_r	CAAGTGCATGTGTTATTCGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-