KIAA0108

Product ID	ORK00411
Accession No	D14696
Description	lysosomal protein transmembrane 4 alpha
Clone name	ha00623
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (1402 bp) Predicted protein sequence (254 aa)
HaloTag ORF Clone	FHC00411
Flexi ORF Clone	FXC00411
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0108 by Kazusa Mouse cDNA Project

Length: 1402 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	554 bp
Genome contig ID	gi89161199r_19995893
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGATAAGATGATTTAATAAATCTCTTTAAATACTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGGGTTGTCATTGTTTTTAATCCGTTACTTTTTTTTAAGATAAACCCTGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	r	20095893	20114908	7	100.0	Perfect prediction

Length: 254 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15012	9.6e-96	100.0	Lysosomal-assoc...	Homo sapiens
AAH03158	1.8e-95	99.6	Lysosomal prote...	Homo sapiens
Q4R4I5	3.7e-95	99.1	Lysosomal-assoc...	Macaca fascicularis
Q6QRN8	1.1e-94	98.3	Lysosomal-assoc...	Bos taurus
Q5EI37	2.6e-94	97.9	Lysosomal-assoc...	Gekko japonicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004687	58	254	PF03821	Golgi 4-transmembrane spanning transporter
HMMTigr	IPR004687	31	254	TIGR00799	Golgi 4-transmembrane spanning transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	50	TIILGTWYMVVNLLMAILLTVEV	72	PRIMARY	23
	2	98	ADNACVLFAVSVLMFIISSMLVY	120	PRIMARY	23
	3	130	LIPFFCYRLFDFVLSCLVAISSL	152	PRIMARY	23
	4	181	CLLFIVLVFFALFIIFKAYLINC	203	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TTTGTTACTTGCTACCCTGAG
Primer_r	CAACCATCATCTTCCACAGTC
PCR product length	148 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles